Role of ghrelin on growth hormone/insulin-like growth factor-1 axis during endotoxemia

Author(s)

Faim, Felipe ^[1] ; Passaglia, Patricia ^[1] ; Batalhao, Marcelo ^[2] ; Lacchini, Riccardo ^[3] ; Stabile, Angelita Maria ^[2] ; Carnio, Evelin Capellari ^[2]

Affiliation

^[1] Univ Sao Paulo, Med Sch Ribeirao Preto, Dept Physiol, Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Nursing Sch Ribeirao Preto, Dept Gen & Specialized Nursing, Ribeirao Preto, SP - Brazil

^[3] Univ Sao Paulo, Nursing Sch Ribeirao Preto, Dept Psychiat & Human Sci, Ribeirao Preto, SP - Brazil

• Document type: Journal article
• Source: GROWTH HORMONE & IGF RESEARCH; v. 48-49, p. 36-44, OCT-DEC 2019.
• Web of Science Citations: 0

Abstract

Objective: To investigate the anti-inflammatory property of ghrelin treatment on the Growth Hormone (GH)/Insulin-like Growth Factor-I (IGF-1) axis in Wistar rats that have undergone endotoxemia. Design: In this randomized animal study, lipopolysaccharide (LPS) (5 mg/kg; intraperitoneal) was administered to induce endotoxemia, and ghrelin (15 nmol/kg; endovenous) was injected simultaneously. Blood and liver samples were collected 2 h, 6 h and 12 h after LPS administration for analysis. Measurements: Tumor necrosis factor alpha (TNF-alpha), interleukin (IL)-1, beta (IL-1 beta), and IL-6 from both blood and liver were determined by ELISA assay. Serum nitrate was determined by chemiluminescense. Growth hormone receptor (GHR) and growth hormone secretagogue receptor 1a (GHSR-1a) were determined by western blotting. GHR mRNA and IGF-1 mRNA were determined by RT-PCR. Results: LPS administration induced a decrease in IGF-1 and GH serum levels, characterizing GH/IGF-1 axis disruption. Ghrelin treatment attenuated the decrease of serum levels of IGF-1 as well as the increase of TNF-alpha, IL-1 beta, IL-6 and nitrate induced by LPS. The increase of induced GHSR-1a protein expression seen in the LPS group after 2 h remained until 6 h after ghrelin treatment. However, attenuation of the circulating IGF-1 decrease by ghrelin treatment was not accompanied by changes in GHR protein expression nor GHR and IGF-1 gene expression. Conclusion: Ghrelin was able to attenuate changes in the GH/IGF-1 axis observed during systemic inflammation, which may be due to the modulation of pro-inflammatory mediators release. (AU)

• FAPESP's process: 15/09857-7 - Central effect of angiotensin (1-7) on the vasopressin release during endotoxemic shock.
• Grantee: Evelin Capellari Cárnio
• Support Opportunities: Regular Research Grants
• FAPESP's process: 16/17681-9 - Pathophysiological changes during systemic inflammation
• Grantee: Luiz Guilherme de Siqueira Branco
• Support Opportunities: Research Projects - Thematic Grants