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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Designing biocompatible and multicolor fluorescent hydroxyapatite nanoparticles for cell-imaging applications

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Machado, T. R. [1] ; Leite, I. S. [2] ; Inada, N. M. [2] ; Li, M. S. [2] ; da Silva, J. S. [1] ; Andres, J. [3] ; Beltran-Mir, H. [4] ; Cordoncillo, E. [4] ; Longo, E. [1]
Total Authors: 9
[1] Univ Fed Sao Carlos UFSCar, Dept Quim, CDMF, BR-13565905 Sao Carlos, SP - Brazil
[2] Univ Sao Paulo, Inst Fis Sao Carlos, BR-13560970 Sao Carlos, SP - Brazil
[3] Univ Jaume 1, Dept Quim Fis & Analit 1, Castellon de La Plana 12071 - Spain
[4] Univ Jaume 1, Dept Quim Inorgan & Organ, Castellon de La Plana 12071 - Spain
Total Affiliations: 4
Document type: Journal article
Web of Science Citations: 1

In recent years, there has been a growing effort toward the synthesis, engineering and property tuning of biocompatible nanoparticles (NPs) that can be detected by confocal microscopy and then used as fluorescent probes. Defect-related fluorescent hydroxyapatite (HA) is attracting considerable attention as a suitable material for cell-imaging owing to its excellent biocompatibility, biodegradability, easy cell internalization capability, and its stable and intense blue fluorescence. Although the self-activated fluorescence of HA is advantageous, as it avoids the use of lanthanide dopants, organic dyes, or the need to be combined with other fluorescent inorganic nanocrystals, its preparation by simple procedures with fine control of the defects which govern this property remains challenging. In this study, we propose a new, simple, and cost-effective strategy of fluorescence imaging using HA nanorods (HAnrs) obtained by chemical precipitation followed by heat treatment at relative low temperature (350 degrees C) without using any sophisticated equipment or inorganic/organic additives. Structural, compositional, and morphological analysis, as well as a cytotoxicity assay, are described in detail. The fluorescence characterization of HAnrs shows an intense bluish-white broad-band emission (lambda(max) = 535 nm), and the defects which cause this behavior are studied by temperature-dependent photoluminescence measurements (38-300 K). Moreover, the high density of defects in heat-treated HA leads to tunable fluorescent property (lambda(max) = 399-650 nm) across the entire visible spectrum as a function of the excitation wavelength (lambda(exc) = 330-630 nm) with the potential for further multicolor imaging applications. Labeling results by confocal microscopy show that HAnr co-cultured with human dermal fibroblast cell line exhibit fluorescence signals in the cells even after 48 h of incubation with no evident cytotoxic effects. Therefore, heat-treated fluorescent HAnr can be utilized for tracking and monitoring cells and is a safe alternative for the traditional probes used in bioimaging procedures. (C) 2019 Elsevier Ltd. All rights reserved. (AU)

FAPESP's process: 13/07276-1 - CEPOF - Optics and Photonic Research Center
Grantee:Vanderlei Salvador Bagnato
Support type: Research Grants - Research, Innovation and Dissemination Centers - RIDC
FAPESP's process: 09/54035-4 - Facility for advanced studies of biosystems and nanostructured materials
Grantee:Igor Polikarpov
Support type: Multi-user Equipment Program
FAPESP's process: 13/11144-3 - Study, Characterization and Properties of Multifunctional Orthophosphates
Grantee:Thales Rafael Machado
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 13/07296-2 - CDMF - Center for the Development of Functional Materials
Grantee:Elson Longo da Silva
Support type: Research Grants - Research, Innovation and Dissemination Centers - RIDC