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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Non-random distribution of microsatellite motifs and (TTAGGG)n repeats in the monkey frog Pithecopus rusticus (Anura, Phyllomedusidae) karyotype

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Author(s):
Julia R. Ernetti [1] ; Camilla B. Gazolla [2] ; Shirlei M. Recco-Pimentel [3] ; Elaine M. Luca ; Daniel P. Bruschi [5]
Total Authors: 5
Affiliation:
[1] Universidade Comunitária da Região de Chapecó. Programa de Pós-graduação em Ciências Ambientais. Área de Ciências Exatas e Ambientais - Brasil
[2] Universidade Federal do Paraná. Programa de Pós-graduação em Genética. Departamento de Genética - Brasil
[3] Universidade Estadual de Campinas. Departamento de Biologia Estrutural e Funcional - Brasil
[5] Universidade Federal do Paraná. Programa de Pós-graduação em Genética. Departamento de Genética - Brasil
Total Affiliations: 5
Document type: Journal article
Source: GENETICS AND MOLECULAR BIOLOGY; v. 42, n. 4 2020-01-13.
Abstract

Abstract The monkey frog, Pithecopus rusticus (Anura, Phyllomedusidae) is endemic to the grasslands of the Araucarias Plateau, southern Brazil. This species is known only from a small population found at the type locality. Here, we analyzed for the first time the chromosomal organization of the repetitive sequences, including seven microsatellite repeats and telomeric sequences (TTAGGG)n in the karyotype of the species by Fluorescence in situ Hybridization. The dinucleotide motifs had a pattern of distribution clearly distinct from those of the tri- and tetranucleotides. The dinucleotide motifs are abundant and widely distributed in the chromosomes, located primarily in the subterminal regions. The tri- and tetranucleotides, by contrast, tend to be clustered, with signals being observed together in the secondary constriction of the homologs of pair 9, which are associated with the nucleolus organizer region. As expected, the (TTAGGG)n probe was hybridized in all the telomeres, with hybridization signals being detected in the interstitial regions of some chromosome pairs. We demonstrated the variation in the abundance and distribution of the different microsatellite motifs and revealed their non-random distribution in the karyotype of P. rusticus. These data contribute to understand the role of repetitive sequences in the karyotype diversification and evolution of this taxon. (AU)

FAPESP's process: 16/07717-6 - Classical and molecular markers for chromosomal studies in anurans
Grantee:Shirlei Maria Recco-Pimentel
Support Opportunities: Regular Research Grants