Carnosine treatment during human semen processing by discontinuous density gradient

Author(s)

Adami, Luana Nayara Gallego ^[1] ; de Lima, Bruna Tuani ^[1] ; Andretta, Rhayza Roberta ^[1] ; Bertolla, Ricardo Pimenta ^[1] ; Nichi, Marcilio ^[1]

Affiliation

^[1] Univ Fed Sao Paulo, Dept Surg, Div Urol, Human Reprod Sect, Rua Embau 231, BR-04039060 Sao Paulo, SP - Brazil

• Document type: Journal article
• Source: ANDROLOGIA; v. 52, n. 2 DEC 2019.
• Web of Science Citations: 0

Abstract

The aim of this article was to evaluate the effects of different concentrations of carnosine added during human semen processing. Semen samples from 34 patients were submitted to processing by discontinuous density gradient centrifugation without (control) or with different concentrations of carnosine supplementation as follows: (a) 20 mM of carnosine supplementation on the layers of Percoll; and (b) 50 mM carnosine supplementation. Sperm samples were then washed with human tubal fluid medium and evaluated according to sperm kinetics and functional assessment. For statistical analysis, data were evaluated by a general linear model or a Friedman test, whenever appropriate. The 50 mM carnosine supplementation led to improved sperm mitochondrial activity when compared to untreated samples. Motility variables, such as percentage of motile and progressively motile spermatozoa, average path velocity, straight line velocity, curvilinear velocity and linearity, showed an improvement after semen processing irrespective of carnosine supplementation. Both concentrations of carnosine increased the beat-cross frequency (BCF) when compared to samples before processing. We conclude that carnosine supplementation in semen samples benefits sperm mitochondrial activity and BCF. (AU)

• FAPESP's process: 16/13822-7 - The effect of treatment with different concentration of antioxidant Carnosine during processing of human semen samples
• Grantee: Luana Nayara Gallego Adami
• Support Opportunities: Scholarships in Brazil - Master