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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Intraspecific Variation within the Utricularia amethystina Species Morphotypes Based on Chloroplast Genomes

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Author(s):
Silva, Saura R. [1] ; Pinheiro, Daniel G. [1] ; Penha, Helen A. [1] ; Plachno, Bartosz J. [2] ; Michael, Todd P. [3] ; Meer, Elliott J. [4] ; Miranda, Vitor F. O. [5] ; Varani, Alessandro M. [1]
Total Authors: 8
Affiliation:
[1] Univ Estadual Paulista Unesp, Dept Tecnol, Fac Ciencias Agr & Vet, BR-14884900 Sao Paulo - Brazil
[2] Jagiellonian Univ Krakow, Fac Biol, Inst Bot, Dept Plant Cytol & Embryol, PL-30387 Krakow - Poland
[3] J Craig Venter Inst, La Jolla, CA 92037 - USA
[4] 10X Genomics, Pleasanton, CA 94566 - USA
[5] Univ Estadual Paulista Unesp, Fac Ciencias Agr & Vet, Dept Biol Aplicada Agr, BR-14884900 Sao Paulo - Brazil
Total Affiliations: 5
Document type: Journal article
Source: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES; v. 20, n. 24 DEC 2019.
Web of Science Citations: 0
Abstract

Utricularia amethystina Salzm. ex A.St.-Hil. \& Girard (Lentibulariaceae) is a highly polymorphic carnivorous plant taxonomically rearranged many times throughout history. Herein, the complete chloroplast genomes (cpDNA) of three U. amethystina morphotypes: purple-, white-, and yellow-flowered, were sequenced, compared, and putative markers for systematic, populations, and evolutionary studies were uncovered. In addition, RNA-Seq and RNA-editing analysis were employed for functional cpDNA evaluation. The cpDNA of three U. amethystina morphotypes exhibits typical quadripartite structure. Fine-grained sequence comparison revealed a high degree of intraspecific genetic variability in all morphotypes, including an exclusive inversion in the psbM and petN genes in U. amethystina yellow. Phylogenetic analyses indicate that U. amethystina morphotypes are monophyletic. Furthermore, in contrast to the terrestrial Utricularia reniformis cpDNA, the U. amethystina morphotypes retain all the plastid NAD(P)H-dehydrogenase (ndh) complex genes. This observation supports the hypothesis that the ndhs in terrestrial Utricularia were independently lost and regained, also suggesting that different habitats (aquatic and terrestrial) are not related to the absence of Utriculariandhs gene repertoire as previously assumed. Moreover, RNA-Seq analyses recovered similar patterns, including nonsynonymous RNA-editing sites (e.g., rps14 and petB). Collectively, our results bring new insights into the chloroplast genome architecture and evolution of the photosynthesis machinery in the Lentibulariaceae. (AU)

FAPESP's process: 13/25164-6 - Genomics and transcriptomics of Utricularia reniformis (Lentibulariaceae): an evolutive and functional approach
Grantee:Alessandro de Mello Varani
Support Opportunities: Regular Research Grants
FAPESP's process: 18/02285-6 - Comparative genomics and molecular evolution of carnivorous plants of the genus Utricularia (Lentibulariaceae)
Grantee:Saura Rodrigues da Silva
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 13/05144-0 - Identification and expression of genes related to floral pigmentation in Utricularia (Lentibulariaceae) with an evolutionary approach
Grantee:Vitor Fernandes Oliveira de Miranda
Support Opportunities: Regular Research Grants