Immunolocalization of antioxidant enzymes in testis of rams submitted to long-term heat stress

Author(s)

dos Santos Hamilton, Thais Rose ^[1] ; Duarte, Gabriela Esteves ^[1] ; Visintin, Jose Antonio ^[1] ; Ortiz D'Avila Assumpcao, Mayra Elena ^[1]

Affiliation

^[1] Univ Sao Paulo, Sch Vet Med & Anim Sci, Anim Reprod Dept, Av Prof Dr Orlando Marques de Paiva 87, BR-05508270 Sao Paulo, SP - Brazil

• Document type: Journal article
• Source: ZYGOTE; v. 27, n. 6, p. 432-435, DEC 2019.
• Web of Science Citations: 0

Abstract

Long-term heat stress (HS) induced by testicular insulation generates oxidative stress (OS) on the testicular environment; consequently activating antioxidant enzymes such as superoxide dismutase (SOD), glutathione reductase (GR) and glutathione peroxidase (GPx). The aim of this work was to immunolocalize antioxidant enzymes present in different cells within the seminiferous tubule when rams were submitted to HS. Rams were divided into control (n = 6) and treated group (n = 6), comprising rams subjected to testicular insulation for 240 h. After the testicular insulation period, rams were subjected to orchiectomy. Testicular fragments were submitted to immunohistochemistry for staining against SOD, GR and GPx enzymes. We observed immunolocalization of GPx in more cell types of the testis after HS and when compared with other enzymes. In conclusion, GPx is the main antioxidant enzyme identified in testicular cells in an attempt to maintain oxidative balance when HS occurs. (AU)

• FAPESP's process: 11/11231-8 - Study of factors involved in DNA fragmentation of spermatozoa in ram.
• Grantee: Thais Rose dos Santos Hamilton
• Support Opportunities: Scholarships in Brazil - Doctorate