DNA modifications can provoke changes in reproduction related processes, and the sperm chromatin integrity guarantees the delivery of paternal genetic information effectively. DNA injuries could be due to factors such as failure protamination, apoptosis and result from reactive oxygen species activity. This DNA damage in sperm can cause male infertility. There is little information on fragmentation rates and the correlation between sperm DNA fragmentation and male reproductive efficiency in ram. This work will be undertaken to evaluate the DNA fragmentation rate, type of protamine present in sperm and establish a correlation between the type of protamine and the sperm DNA fragmentation rate in ram. For this purpose we will determine the gene expression of protamine 1 and 2, and the transition proteins 1 and 2, and evaluate the susceptibility of sperm DNA fragmentation, as well as the its correlation to oxidative stress, apoptosis and packaging sperm chromatin damage.
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