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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Antiviral activity of curcumin-nanoemulsion associated with photodynamic therapy in vulvar cell lines transducing different variants of HPV-16

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Author(s):
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do Bonfim, Caroline Measso [1] ; Monteleoni, Leticia Figueiredo [1] ; Calmon, Marilia de Freitas [1] ; Candido, Natalia Maria [1] ; Scarin Provazzi, Paola Jocelan [1] ; Lino, Vanesca de Souza [2] ; Rabachini, Tatiana [3] ; Sichero, Laura [4] ; Villa, Luisa Lina [5, 4] ; Quintana, Silvana Maria [6] ; dos Santos Melli, Patricia Pereira [6] ; Primo, Fernando Lucas [7] ; Amantino, Camila Fernanda [8] ; Tedesco, Antonio Claudio [8] ; Boccardo, Enrique [2] ; Rahal, Paula [1]
Total Authors: 16
Affiliation:
[1] Sao Paulo State Univ UNESP, Lab Genom Studies, Sao Paulo - Brazil
[2] Univ Sao Paulo, Inst Biomed Sci, Dept Microbiol, Sao Paulo - Brazil
[3] Univ Bern, Inst Pharmacol, Bern - Switzerland
[4] Inst Canc Estado Sao Paulo, Ctr Translat Res Oncol, Sao Paulo - Brazil
[5] Univ Sao Paulo, Sch Med, Dept Radiol & Oncol, Sao Paulo - Brazil
[6] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Gynecol & Obstet, Ribeirao Preto, SP - Brazil
[7] Univ Estadual Paulista Julio de Mesquita Filho UN, Fac Pharmaceut Sci Araraquara, Dept Bioproc & Biotechnol, Araraquara, SP - Brazil
[8] Univ Sao Paulo, Fac Philosophy Sci & Languages Ribeirao Preto, Ctr Nanotechnol & Tissue Engn, Dept Chem, Ribeirao Preto, SP - Brazil
Total Affiliations: 8
Document type: Journal article
Source: ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY; v. 48, n. 1, p. 515-524, JAN 1 2020.
Web of Science Citations: 0
Abstract

Vulvar intraepithelial neoplasia (VIN) is associated with human papillomavirus (HPV) infection. Curcumin is a natural bioactive compound with antineoplastic properties. The use of nanoparticles containing curcumin could allow a better performance of this compound in therapies. So, VIN biopsies were collected and HPV DNA detection was performed by PCR, positive samples were genotyped by Restriction Fragment Length Polymorphism (RFLP) and HPV-16 variants were determined by sequencing. HPV-16 positive vulva carcinoma cells (A431) were transduced with E-P and E-350G HPV-16 E6 variants. The viability of the transduced cells treated with nanoemulsions was determined by MTT assay. Besides, apoptosis was evaluated by enzymatic activity of Caspase-3/7. The cell viability assay showed that both the empty nanoemulsion (NE-V) and the nanoemulsion of curcumin (NE-CUR) had little effect on cell viability as compared to control cells. Additionally, we observed that cells irradiated in the presence of NE-CUR presented 90% of cell death. The apoptosis assay further revealed a significant increase in the activity of caspases 3 and 7 in A431 cells expressing both HPV-16 E6 variants after treatment with NE-CUR. Finally, we submitted the HPV transduced A431 cells to organotypic cultures and observed that the combination of treatments affected tissue architecture with evident signals of tissue damage. We concluded that nanoemulsions attain good biocompatibility, since no cytotoxicity was observed and NE-CUR associated with photoactivation showed promising results, leading to death only in cells subjected to irradiation. This drug delivery system associated with photodynamic therapy may become promising in the treatment of vulva lesions. (AU)

FAPESP's process: 14/02064-9 - Analysis of the antiviral action of the curcumin natural compound associated with nanoparticles in HPV-16 positive and negative vulvar carcinoma cell lines
Grantee:Caroline Measso Do Bonfim
Support Opportunities: Scholarships in Brazil - Post-Doctoral