Metalloproteomic approach of mercury -binding proteins in liver and kidney tissues of Plagioscion squamosissimus (corvina) and Colossoma macropomum (tambaqui) from Amazon region: Possible identification of mercury contamination biomarkers

Author(s):Show less -

Bittarello, Alis Correia ^[1] ; Souza Vieira, Jose Cavalcante ^{[2, 3]} ; Braga, Camila Pereira ^[4] ; Bataglioli, Izabela da Cunha ^[1] ; de Oliveira, Grasieli ^[2] ; Rocha, Leone Campos ^[1] ; Zara, Luiz Fabricio ^[5] ; Rabelo Buzalaf, Marilia Afonso ^[6] ; Silva de Oliveira, Lincoln Carlos ^[3] ; Adamec, Jiri ^[4] ; Padilha, Pedro de Magalhaes ^{[2, 1]}

Affiliation

^[1] Sao Paulo State Univ, UNESP, Sch Vet Med & Anim Sci, Botucatu - Brazil

^[2] Sao Paulo State Univ, UNESP, Inst Biosci, Botucatu - Brazil

^[3] Univ Fed Mato Grosso do Sul, Campo Grande UFMS, Inst Chem INQUI, Campo Grande, MS - Brazil

^[4] Univ Nebraska UNL, Lincoln, NE - USA

^[5] Univ Brasilia UNB, Coll Planaltina, Dist Fed - Brazil

^[6] Univ Sao Paulo, Bauru - Brazil

• Document type: Journal article
• Source: Science of The Total Environment; v. 711, APR 1 2020.
• Web of Science Citations: 0

Abstract

Fish is an important source of protein, vitamins, and minerals. However, this food is also a major source of human exposure to toxic contaminants such as mercury. Thus, this paper aimed to evaluate mercury-binding proteins for possible application as biomarkers of mercury contamination in hepatic and renal tissues of Plagioscion squamosissimus (carnivorous fish) and Colossoma macropomum (omnivorous fish) from the Amazon region using metalloproteomic approach. The proteome of hepatic and renal tissues of fish species was separated by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE), and the mercury concentrations in protein spots were determined by graphite furnace atomic absorption spectrometry (GFAAS). Finally, the protein spots associated to mercury were characterized by electrospray ionization mass spectrometry (ESI-MS/MS). The activity of antioxidant enzymes (SOD, CAT, GPx, and GST) and lipid peroxidation (LPO) were also determined. The results showed that the highest concentrations of mercury were found in the carnivorous species (P. squamosissimus) and that the accumulation pattern of this metal was higher in hepatic tissues than in renal tissues for both species. A tendency was observed for greater enzymatic activity in the hepatic and renal tissues of P. squamosissimus, the species with the highest concentration of mercury. Only GPx activity in the kidney and GST in the liver were lower for the P. squamosissimus species, and this finding can be explained by the interaction of mercury with these enzymes. The data obtained by ESI-MS/MS allowed for the characterization of the protein spots associated to mercury, revealing proteins involved in energy metabolism, biomolecules transport, protein synthesis and degradation, cell differentiation, gene regulation, and the antioxidant system. The results obtained in the present study can contribute to understanding the physiological processes underlying mercury toxicity and have provided new perspectives on possible candidates for mercury contamination biomarkers in fish. (C) 2019 Elsevier B.V. All rights reserved. (AU)

• FAPESP's process: 14/02668-1 - Utilization of metalloproteomic strategies for metallothionein evaluation in liver tissue samples of fishes collected in the reservoir of Ahe Jirau - Madeira River Basin, RO, Brazil
• Grantee: José Cavalcante Souza Vieira
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 16/19404-2 - Mercury toxicity biomarker characterization in fish from Basin of the Madeira River-Rondônia using metalloproteomic strategies
• Grantee: Pedro de Magalhães Padilha
• Support Opportunities: Regular Research Grants