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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Anti-EGFR-Coated Gold Nanoparticles In Vitro Carry 5-Fluorouracil to Colorectal Cancer Cells

Full text
Author(s):
Liszbinski, Raquel B. [1] ; Romagnoli, Graziela G. [1] ; Gorgulho, Carolina M. [1] ; Basso, Caroline R. [2] ; Pedrosa, Valber A. [2] ; Kaneno, Ramon [1]
Total Authors: 6
Affiliation:
[1] UNESP, Dept Microbiol & Immunol, Inst Biosci, BR-18618000 Botucatu, SP - Brazil
[2] UNESP, Dept Chem & Biochem, Inst Biosci, BR-18618000 Botucatu, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: MATERIALS; v. 13, n. 2 JAN 2 2020.
Web of Science Citations: 7
Abstract

The aim of the current study is to present a strategy to improve the efficiency of 5-fluorouracil (5-FU), which is widely used as antineoplastic agent against solid tumors-based on the use of gold nanocarriers to overcome the resistance of colorectal cancer cells. 5-FU was loaded on gold nanoparticles (AuNP) coated with anti-EGFR antibodies in order to target them towards colorectal cancer cells that overexpress epidermal growth factor receptors (EGFR). Physicochemical characterization has shown that AuNP size was approximately 20 nm and that AuNP functionalization led to spherical nanoparticles. Flow cytometry allowed observing that some compounds synthesized by our research group have induced apoptosis/necrosis and impaired the proliferation of colon cancer cell lines `HCT-116 ` and `HT-29 `. The antibody/drug combination in AuNP (AuNP 5FU EGFR) has improved the apoptosis rate and impaired cell proliferation in both cell lines, regardless of the exposure time. Overall, these results have shown that AuNP functionalization with monoclonal antibodies focused on delivering 5-FU to tumor cells is an exciting strategy against colorectal cancer. (AU)

FAPESP's process: 15/26729-2 - TRANSPORT OF 5-FLUOROURACIL BY FUNCIONALIZED GOLD NANOPARTICLES WITH ANTIBODIES AGAINST EPIDERMAL GROWTH FACTOR RECEPTORS (EGFR AND HER2)
Grantee:Raquel Bester Liszbinski
Support Opportunities: Scholarships in Brazil - Master
FAPESP's process: 19/13411-5 - Development of microfluidic device for liquid biopsy of circulating tumor cells
Grantee:Valber de Albuquerque Pedrosa
Support Opportunities: Regular Research Grants