LMWPTP modulates the antioxidant response and autophagy process in human chronic myeloid leukemia cells

Author(s)

Faria, Alessandra V. S. ^{[1, 2]} ; Clerici, Stefano P. ^[1] ; De Souza Oliveira, Patricia F. ^[1] ; Queiroz, Karla C. S. ^[1] ; Peppelenbosch, Maikel P. ^[2] ; Ferreira-Halder, Carmen V. ^[1]

Affiliation

^[1] Univ Estadual Campinas, Dept Biochem, Tissue Biol, UNICAMP, Campinas, SP - Brazil

^[2] Univ Med Ctr Rotterdam, Dept Gastroenterol, Hepatol, Erasmus MC, Box 2040, NL-3000 CA Rotterdam - Netherlands

• Document type: Journal article
• Source: Molecular and Cellular Biochemistry; v. 466, n. 1-2, p. 83-89, MAR 2020.
• Web of Science Citations: 0

Abstract

In the last decade, several reports highlight the importance of the low molecular weight protein tyrosine phosphatase (LMWPTP) in cancer aggressiveness and resistance. Specifically, in chronic myeloid leukemia, we have reported that high expression of the LMWPTP maintains Src and Bcr-Abl kinases in an activated status and the glucose metabolism is directed to lactate production and, in turn, favor the pentoses pathway (one of the key process for antioxidant and protective responses). In this present study, we investigated the possible correlation between the LMWPTP and autophagy. In resistant chronic myeloid leukemia cells, the antioxidant response is supported by the glycolytic metabolism and antioxidant enzymes such as SOD and catalase, both favored by the LMWPTP. Therefore, when the cells were challenged by hydrogen peroxide treatment, the LMWPTP level goes down as well as SOD, and in turn, autophagy process was stimulated. The findings presented here reveal a novel aspect by which LMWPTP cooperates for the resistance of CML towards stressor stimuli. (AU)

• FAPESP's process: 18/03593-6 - Tumor-educated platelets and metastasis: relevance of LMWPTP and extracellular vesicles in Colorectal Cancer
• Grantee: Stefano Piatto Clerici
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 17/08119-8 - Hematogenous tumor metastasis in colon rectal cancer cells: influence of LMWPTP and 3-bromopyruvate
• Grantee: Alessandra Valéria de Sousa Faria
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 15/20412-7 - Low molecular weight protein tyrosine phosphatase in colorectal cancer: from the bench to product generation
• Grantee: Carmen Veríssima Ferreira
• Support Opportunities: Research Projects - Thematic Grants