| Full text | |
| Author(s): |
do Amaral, Jonatas Bussador
[1]
;
Bloise, Antonio Carlos
[2]
;
Franca, Carolina Nunes
[3]
;
Perez-Novo, Claudina
[4]
;
Machado-Santelli, Glaucia Maria
[5]
;
Alencar, Adriano Mesquita
[2]
;
Pezato, Rogerio
[1]
Total Authors: 7
|
| Affiliation: | [1] Univ Fed Sao Paulo, Dept Otorhinolaryngol Head & Neck Surg, ENT Res Lab, Rua Coronel Lisboa 958, BR-04020041 Sao Paulo, SP - Brazil
[2] Univ Sao Paulo, Inst Phys, Rua Matao, Travessa R 187, BR-05508090 Sao Paulo, SP - Brazil
[3] Univ Santo Amara, Pos Grad Ciencias Saude, Rua Eneas de Siqueira Neto, 340 Jardim Imbuias, BR-04829300 Sao Paulo, SP - Brazil
[4] Wilrijk Univ Antwerp, Lab Prot Chem Prote & Epigenet Signalling, Dept Biomed Sci, Campus Drie Eiken, Bldg T, First Floor Univ Pl 1, B-2610 Antwerp - Belgium
[5] Univ Sao Paulo, Dept Cell & Dev Biol, Inst Biomed Sci, Ave Prof Lineu Prestes 1524, BR-05508900 Sao Paulo, SP - Brazil
Total Affiliations: 5
|
| Document type: | Journal article |
| Source: | AURIS NASUS LARYNX; v. 47, n. 1, p. 98-104, FEB 2020. |
| Web of Science Citations: | 0 |
| Abstract | |
Objective: Chronic Rhinosinusitis with Nasal Polyps (CRSwNP) is a disease that features a mechanical dysfunction involving chronic inflammation and altered tissue remodeling. In this study, we aim to evaluate the fibroblast morphology and its cellular traction force in primary fibroblasts cell cultures obtained from both healthy individuals (n = 7) and patients with CRSwNP (n = 8). Methods: Using a Traction-force Microscopy we analyzed parameters of Force/Tension in fibroblasts cultures in both experimental groups. Results: The analysis of the Projected Area of Cell revealed that fibroblasts derived from nasal mucosa of healthy individuals have an area on average 39.24% larger than the fibroblasts obtained from the nasal polyp tissue. We also observed that the parameters directly related to the force of the cell, Max Cumulative Force and Net Contractile Moment, presented a high Force/Tension per unit of area in the fibroblasts derived from the healthy nasal mucosa (on average 41% and 52.54% higher than the fibroblasts of the nasal polyp respectively). Conclusion: Our results demonstrate a cellular mechanism that may be associated with the mechanical dysfunction found in the Nasal Polyp tissue. The weak traction force of nasal polyp-derived fibroblast may, in lower dimensions, impact on the remodeling of nasal mucosa in CRSwNP. (C) 2019 Elsevier B.V. All rights reserved. (AU) | |
| FAPESP's process: | 14/22102-2 - Characterization of cardiomyocytes dynamics by traction force microscopy (TFM) and Speckle |
| Grantee: | Adriano Mesquita Alencar |
| Support Opportunities: | Regular Research Grants |