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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Molecular detection of Hepatozoon spp. in non-hematophagous bats in Brazil

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Author(s):
Perles, Livia [1] ; Ikeda, Priscila [1] ; Francisco, Gabriela de Vasconcellos [1] ; Torres, Jaire Marinho [1, 2, 3] ; de Oliveira, Carina Elisei [2] ; Lourenco, Elizabete Captivo [3] ; Herrera, Heitor Miraglia [2] ; Machado, Rosangela Zacarias [1, 2, 3] ; Andre, Marcos Rogerio [1]
Total Authors: 9
Affiliation:
[1] Univ Estadual Julio de Mesquita Filho, Dept Patol Vet, Lab Imunoparasitol, Jaboticabal - Brazil
[2] Univ Catolica Dom Bosco, Lab Ciencias Ambientais & Sustentabilidade Agr, Campo Grande, MS - Brazil
[3] Univ Estado Rio de Janeiro, Lab Ecol Mamiferos, Rio De Janeiro - Brazil
Total Affiliations: 3
Document type: Journal article
Source: TICKS AND TICK-BORNE DISEASES; v. 11, n. 3 MAY 2020.
Web of Science Citations: 0
Abstract

Even though Hepatozoon spp. has been molecularly detected in several wild animals in Brazil, there is no report on the occurrence of Hepatozoon spp. DNA in bats in Brazil. This study aimed at detecting Hepatozoon, in addition to ectoparasites, in non-hematophagous bats sampled in central-western Brazil using blood smears, hematoxylineosin (HE)-staining liver/spleen preparations and molecular and phylogenetic techniques. A total of 135 spleen, 127 liver, and 133 blood samples were collected from 135 non-hematophagous bats from 12 different species in two different sites in Campo Grande, Mato Grosso do Sul state, in the Brazilian Cerrado region. Spleen and blood DNA samples were submitted to two conventional PCR protocols for Hepatozoon spp. based on 18S rRNA. No Hepatozoon spp. gamonts or meronts were observed in blood smears and HE-stained-liver preparations, respectively. While none of the spleen samples was positive for Hepatozoon spp. in the PCR assays, 5 (3 %) blood samples contained 18S rRNA Hepatozoon DNA, including 2/37 (5 %) Artibeus lituratus, 2/32 (6 %) A. planirostris, and 1/23 (4 %) Platyrrhinus lineatus. Out of 5 bats positive for Hepatozoon spp., 3 were parasitized by either Macronyssidae/Spinturnicidae mites or Streblidae flies. BLAST analysis showed that the sequences detected in bats had > 99 % identity with Hepatozoon sequences detected in amphibians and reptiles from Brazil, including Hepatozoon caimani detected in Caiman crocodiles. The phylogenetic inferences estimated by the Maximum Likelihood and Bayesian methods clustered the Hepatozoon sequences detected in Brazilian bats with those detected in reptiles and amphibians. (AU)

FAPESP's process: 18/02753-0 - ISOLATION AND GENOTYPING OF Bartonella spp. IN DOMESTIC AND WILD RESERVOIR MAMMALS IN BRAZIL
Grantee:Marcos Rogério André
Support Opportunities: Regular Research Grants
FAPESP's process: 19/13232-3 - Bacterial microbiome of non-hematophagous bats' ectoparasites, saliva and feces: a metagenomic approach
Grantee:Priscila Ikeda
Support Opportunities: Scholarships abroad - Research Internship - Doctorate
FAPESP's process: 17/14124-4 - Detection and molecular characterization of Anaplasmataceae, Bartonellaceae and Mycoplasmaceae agents in bats sampled in a peri-urban region of Central-Western Brazil
Grantee:Priscila Ikeda
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 19/15150-4 - Genetic diversity of vector-borne agents in coatis (Nasua nasua) in a periurban region of Central-Western Brazil
Grantee:Lívia Perles
Support Opportunities: Scholarships in Brazil - Doctorate