| Full text | |
| Author(s): Show less - |
Rodrigues Alves, Frederico Rocha
[1, 2]
;
Lira, Bruno Silvestre
[1]
;
Pikart, Filipe Christian
[1]
;
Monteiro, Scarlet Santos
[1]
;
Furlan, Claudia Maria
[1]
;
Purgatto, Eduardo
[3]
;
Pascoal, Grazieli Benedetti
[3, 4]
;
Silva Andrade, Sonia Cristina
[5]
;
Demarco, Diego
[1]
;
Rossi, Magdalena
[1]
;
Freschi, Luciano
[1]
Total Authors: 11
|
| Affiliation: | [1] Univ Sao Paulo, Dept Bot, Sao Paulo, SP - Brazil
[2] Univ Fed Goias, Dept Bot, Goias, Go - Brazil
[3] Univ Sao Paulo, Dept Alimentos & Nutr Expt, Sao Paulo, SP - Brazil
[4] Univ Fed Uberlandia, Curso Grad Nutr, Uberlandia, MG - Brazil
[5] Univ Sao Paulo, Dept Genet & Biol Evolut, Sao Paulo, SP - Brazil
Total Affiliations: 5
|
| Document type: | Journal article |
| Source: | Plant Biotechnology Journal; v. 18, n. 10 APR 2020. |
| Web of Science Citations: | 3 |
| Abstract | |
Photoreceptor engineering has recently emerged as a means for improving agronomically beneficial traits in crop species. Despite the central role played by the red/far-red photoreceptor phytochromes (PHYs) in controlling fruit physiology, the applicability of PHY engineering for increasing fleshy fruit nutritional content remains poorly exploited. In this study, we demonstrated that the fruit-specific overexpression of a constitutively active GAF domain Tyr(252)-to-His PHYB2 mutant version (PHYB2(Y252H)) significantly enhances the accumulation of multiple health-promoting antioxidants in tomato fruits, without negative collateral consequences on vegetative development. Compared with the native PHYB2 overexpression, PHYB2(Y252H)-overexpressing lines exhibited more extensive increments in transcript abundance of genes associated with fruit plastid development, chlorophyll biosynthesis and metabolic pathways responsible for the accumulation of antioxidant compounds. Accordingly, PHYB2(Y252H)-overexpressing fruits developed more chloroplasts containing voluminous grana at the green stage and overaccumulated carotenoids, tocopherols, flavonoids and ascorbate in ripe fruits compared with both wild-type and PHYB2-overexpressing lines. The impacts of PHYB2 or PHYB2(Y252H) overexpression on fruit primary metabolism were limited to a slight promotion in lipid biosynthesis and reduction in sugar accumulation. Altogether, these findings indicate that mutation-based adjustments in PHY properties represent a valuable photobiotechnological tool for tomato biofortification, highlighting the potential of photoreceptor engineering for improving quality traits in fleshy fruits. (AU) | |
| FAPESP's process: | 16/04924-0 - Manipulating phytochrome levels in tomato fruits (Solanum lycopersicum L.) and its impacts on plastid development, physiology and nutritional composition |
| Grantee: | Frederico Rocha Rodrigues Alves |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 18/25774-2 - Overexpression of hyperactive mutant forms of phytochromes B1 and B2 as a means of manipulating tomato quality traits |
| Grantee: | Scarlet Santos Monteiro |
| Support Opportunities: | Scholarships in Brazil - Master |
| FAPESP's process: | 18/16389-8 - Manipulation of light signaling and nitric oxide metabolism as a means of improving tomato quality traits |
| Grantee: | Luciano Freschi |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 16/01128-9 - Light and hormonal regulation of nutritional quality in Solanum lycopersicum |
| Grantee: | Maria Magdalena Rossi |
| Support Opportunities: | Research Projects - Thematic Grants |