Genomic and Clinical Characterization of IMP-1-Producing Multidrug-Resistant Acinetobacter bereziniae Isolates from Bloodstream Infections in a Brazilian Tertiary Hospital

Author(s):Show less -

Brisolla Tavares, Lais Calissi ^{[1, 2]} ; Vieira Cunha, Marcos Paulo ^[3] ; de Vasconcellos, Francielli Mahnic ^[2] ; de Jesus Bertani, Amanda Maria ^[2] ; Franco de Barcellos, Thays Almeida ^{[1, 2]} ; Bueno, Mariana Sardinha ^{[1, 2]} ; Santos, Carla Adriana ^[2] ; Sant'Ana, Daniel Almeida ^[2] ; Ferreira, Adriano Martison ^[4] ; Mondelli, Alessandro Lia ^[4] ; Montelli, Augusto Cezar ^[4] ; Sadatsune, Terue ^[5] ; Sacchi, Claudio Tavares ^[6] ; Goncalves, Claudia Regina ^[6] ; Tiba-Casas, Monique Ribeiro ^[2] ; Camargo, Carlos Henrique ^{[1, 2]}

Affiliation

^[1] Univ Sao Paulo, Fac Med, Sao Paulo - Brazil

^[2] Adolfo Lutz Inst, Ctr Bacteriol, Ave Dr Arnaldo 355, 9 Andar, BR-01246902 Sao Paulo - Brazil

^[3] Univ Sao Paulo, Fac Med Vet & Zootecnia, Sao Paulo - Brazil

^[4] Univ Estadual Paulista, Fac Med Botucatu, Botucatu, SP - Brazil

^[5] Univ Estadual Paulista, Inst Biociencias Botucatu, Botucatu, SP - Brazil

^[6] Adolfo Lutz Inst, Lab Estrateg, Sao Paulo - Brazil

• Document type: Journal article
• Source: MICROBIAL DRUG RESISTANCE; v. 26, n. 11 MAR 2020.
• Web of Science Citations: 1

Abstract

Acinetobacter baumannii is the main species of the Acinetobacter genus; however, non-baumannii Acinetobacter (NBA) species causing infections have been described for the past years, as well as antimicrobial resistance. In this study, we describe the occurrence of two multidrug-resistant (MDR) IMP-1-producing Acinetobacter bereziniae isolates recovered from bloodstream infections in different patients but in the same intensive care unit among 134 carbapenem-resistant Acinetobacter screened. Antimicrobial susceptibility testing revealed resistance to carbapenems, extended spectrum, and antipseudomonad cephalosporins, amikacin, and trimethoprim-sulfamethoxazole. Both A. bereziniae isolates shared the same ApaI-pulsed-field gel electrophoresis (PFGE) pattern. Whole-genome sequencing of both isolates revealed that bla(IMP-1) was embedded into an In86 Class I integron carrying also sul1, aac(6 `)-31, and aadA genes. A new sequence type (ST1309 Pasteur) was deposited. The virulence genes lpxC and ompA, seen in A. baumannii, were detected in the A. bereziniae strains. Recognition of A. bereziniae causing invasive MDR infection underscores the role of NBA species as human pathogens especially in at-risk patients. (AU)

• FAPESP's process: 16/20727-0 - Phenotypic and genotypic characterization of aminoglycoside resistance in Acinetobacter baumannii
• Grantee: Daniel Almeida Sant'Ana
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 15/13179-4 - Genetic diversity and antimicrobial susceptibility profile of multidrug-resistant strains of Acinetobacter calcoaceticus-A. baumannii complex in a teaching hospital
• Grantee: Laís Calissi Brisolla Tavares
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 17/16988-6 - Genomic analysis of high-risk clones of Acinetobacter baumannii from different hospitals in Brazil
• Grantee: Carlos Henrique Camargo
• Support Opportunities: Regular Research Grants