| Full text | |
| Author(s): |
Lobo, Alexandre R.
[1]
;
Gaievski, Eduardo H. S.
[1]
;
de Mesquita, Carlos Henrique
[2]
;
De Carli, Eduardo
[1]
;
Teixeira, Pryscila Dryelle S.
[1]
;
Pereira, Rosa M. R.
[3]
;
Borelli, Primavera
[4]
;
de Sa, Lilian R. M.
[5]
;
Colli, Celia
[1]
Total Authors: 9
|
| Affiliation: | [1] Univ Sao Paulo, Fac Pharmaceut Sci, Dept Food & Expt Nutr, Prof Lineu Prestes, 580, Bloco 14, BR-05508900 Sao Paulo, SP - Brazil
[2] Inst Nucl & Energy Res, Radiat Technol Ctr, Av Prof Lineu Prestes 2242, BR-05508900 Sao Paulo, SP - Brazil
[3] Univ Sao Paulo, Clin Hosp, Fac Med, Bone Metab Lab, Rheumatol Div, Av Dr Arnaldo 455, BR-01246903 Sao Paulo, SP - Brazil
[4] Univ Sao Paulo, Fac Pharmaceut Sci, Dept Clin & Toxicol Anal, Av Prof Lineu Prestes, 580, Bloco 17, BR-05508900 Sao Paulo, SP - Brazil
[5] Univ Sao Paulo, Fac Vet Med & Anim Sci, Dept Pathol, Av Prof Dr Orlando Marques de Paiva 87, BR-05508000 Sao Paulo, SP - Brazil
Total Affiliations: 5
|
| Document type: | Journal article |
| Source: | BRITISH JOURNAL OF NUTRITION; v. 123, n. 10, p. 1094-1108, MAY 28 2020. |
| Web of Science Citations: | 0 |
| Abstract | |
The present study reports the effects of a high-fat (HF) diet of over 8 weeks on the Fe status of growing rats. Tissue Fe levels were analysed by atomic absorption spectrophotometry, and whole-body adiposity was measured by dual-energy X-ray absorptiometry. Histopathology and morphometry of adipose tissue were performed. Liver homogenates were used for measuring ferroportin-1 protein levels by immunoblotting, and transcript levels were used for Fe genes measured by real-time PCR. Tissue Fe pools were fit to a compartmental biokinetic model in which Fe was assessed using fourteen compartments and twenty-seven transfer constants (k(j,i) from tissue `i' to tissue `j') adapted from the International Commission on Radiological Protection (ICRP) 69. Ten k(j,i) were calculated from the experimental data using non-linear regression, and seventeen were estimated by allometry according to the formula \$[k\_[i,j]] = a \textbackslash{}times [M<\^{}>b]\$. Validation of the model was carried out by comparing predicted and analysed Fe pool sizes in erythrocytes, the liver and the spleen. Body adiposity was negatively associated with serum Fe levels and positively associated with liver Fe stores. An inferred increase in Fe transfer from bone marrow to the liver paralleled higher hepatic Fe concentrations and ferritin heavy-chain mRNA levels in the HF diet-fed animals, suggesting that liver Fe accumulation occurred at least in part due to a favoured liver erythrocyte uptake. If this feeding condition was to be prolonged, impaired Fe decompartmentalisation may occur, ultimately resulting in dysmetabolic Fe overload. (AU) | |
| FAPESP's process: | 10/05644-5 - Assessment of iron status in rats fed high-fat diets |
| Grantee: | Célia Colli |
| Support Opportunities: | Regular Research Grants |