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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Performance of distinct phenotypic methods for carbapenemase detection: The influence of culture media

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Author(s):
Cordeiro-Moura, Jhonatha Rodrigo [1] ; Correa Fehlberg, Lorena Cristina [1] ; Nodari, Carolina Silva [2] ; de Matos, Adriana Pereira [1] ; Alves, Vinicius de Oliveira [1] ; Cayo, Rodrigo [2, 1] ; Gales, Ana Cristina [1]
Total Authors: 7
Affiliation:
[1] Univ Fed Sao Paulo UNIFESP, Dept Internal Med, Div Infect Dis, Lab Alerta, EPM, Sao Paulo, SP - Brazil
[2] Univ Fed Sao Paulo UNIFESP, ICAQF, DCB, LIB, Setor Biol Mol Microbiol & Imunol, Diadema, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE; v. 96, n. 1 JAN 2020.
Web of Science Citations: 0
Abstract

We evaluated the performance of five phenotypic tests {[}Modified Hodge Test (MHT); combined-disk test (CDT) using phenylboronic acid, EDTA, and cloxacillin; CarbaNP and CarbAcinetoNP; Blue-Carba, Carbapenembac (TM) and Carbapenembac Metallo (TM)] for carbapenemase detection in Gram-negative bacilli (GNB). A total of 73 carbapenemase producers and 27 non-carbapenemase producers were tested. All GNB were subcultured onto Mueller-Hinton agar (MHA), MacConkey agar (MAC), and sheep blood agar (SBA). High sensitivity (100%) and specificity (100%) was observed for MHA using CarbaNP, Blue-Carba, and Carbapenembac (TM). The sensitivity and specificity of CarbaNP (98.6%/100%), Blue-Carba (97.1%/91.0%), and Carbapenembac (TM) (100%/96.5%) were slightly lower for SBA. In contrast, unacceptable sensitivity rates of CarbaNP (71.1%) and Blue-Carba (66.6%), but not Carbapenembac (TM) (97.3%), were observed for MAC. The colorimetric methods showed high sensitivity and specificity to detect carbapenemase production from isolates grown on MHA or SBA. However, colonies obtained from MAC must not be tested for carbapenemase detection by colorimetric methods. (C) 2019 Elsevier Inc. All rights reserved. (AU)

FAPESP's process: 14/12224-3 - Evaluation of the mechanisms of resistance to beta-lactams among Burkholderia cenocepacia clinical isolates recovered from bloodstream infections
Grantee:Lorena Cristina Corrêa Fehlberg
Support Opportunities: Scholarships in Brazil - Post-Doctoral