| Full text | |
| Author(s): |
Vinueza Galarraga, Julio Cesar
[1, 2]
;
Barud, Hernane Da Silva
[3]
;
Dos Santos, Fernanda Kolenyak
[4]
;
Davanso, Maisa
[5]
;
Paz-Cedeno, Fernando Roberto
[1]
;
De Paula, Ariela Veloso
[1]
;
Monti, Rubens
[1]
;
Masarin, Fernando
[1]
Total Authors: 8
|
| Affiliation: | [1] Sao Paulo State Univ UNESP, Sch Pharmaceut Sci FCF, Dept Bioproc Engn & Biotechnol, BR-14800903 Araraquara, SP - Brazil
[2] Ecuador Pontificia Univ Catolica Ecuador, Fac Ciencias Exactas & Nat, Quito 17012184 - Ecuador
[3] Araraquara Univ Ctr UNIARA, Biopolymers & Biomat Lab, BR-14801320 Araraquara, SP - Brazil
[4] Univ Guayaquil, Fac Ciencias Quim, Guayaquil 2391004 - Ecuador
[5] Sao Paulo State Univ UNESP, Inst Chem, BR-14800900 Araraquara, SP - Brazil
Total Affiliations: 5
|
| Document type: | Journal article |
| Source: | CELLULOSE CHEMISTRY AND TECHNOLOGY; v. 54, n. 3-4, p. 275-283, MAR-APR 2020. |
| Web of Science Citations: | 0 |
| Abstract | |
Peroxidases are homoproteins that catalyze redox processes, generating free radicals and polyaromatic products insoluble in water, facilitating their removal from the aqueous medium. The objective of this research was to extract the peroxidases of soybean and chayote, immobilize these enzymes on a highly activated bacterial cellulose (BC) support and use the derivatives obtained for discoloration of bromophenol blue. The amination of soluble peroxidases was realized in ethylenediamine buffer, at pH 4.75, and 10 and 50 mM carbodiimide. Amined peroxidases of 10 and 50 mM were covalently immobilized on the BC-glyoxyl support, with recovered activity of 82% for the derivative BC-Gly-S-NH2 50 mM and 92% for the derivative BC-Gly-Ch-NH2 50 mM. Total discoloration of bromophenol blue was obtained in 60 min, using the four amino derivatives. The derivatives were reused for five cycles and they maintained an average of 40% of their catalytic properties, suggesting that these products are suitable as a low-cost alternative for wastewater treatment and other industrial processes. (AU) | |
| FAPESP's process: | 14/12563-2 - Agro industrial residues as solid support for immobilization and stabilization of enzymes of biotechnological interest |
| Grantee: | Rubens Monti |
| Support Opportunities: | Regular Research Grants |