Comparison of antibody repertories against Staphylococcus aureus in healthy and infected dairy cows with a distinct mastitis history and vaccinated with a polyvalent mastitis vaccine

Author(s):Show less -

Cunha, A. F. ^{[1, 2]} ; Andrade, H. M. ^[3] ; Souza, F. N. ^{[1, 2, 4, 5]} ; Fialho Junior, L. C. ^[3] ; Rosa, D. L. S. O. ^{[1, 2]} ; Ramos Sanchez, E. M. ^{[6, 7]} ; Gidlund, M. ^[8] ; Goto, H. ^[6] ; Brito, M. A. V. P. ^[9] ; Guimaraes, A. S. ^[10] ; Lage, A. P. ^[1] ; Reis, L. C. ^[6] ; Della Libera, A. M. M. P. ^[4] ; Heinemann, M. B. ^[11] ; Cerqueira, M. M. O. P. ^[2]

Affiliation:Show less -

^[1] Univ Fed Minas Gerais, Dept Med Vet Prevent, Escola Vet, BR-31270010 Belo Horizonte, MG - Brazil

^[2] Univ Fed Minas Gerais, Dept Tecnol & Inspecao Prod Origem Anim, Escola Vet, BR-31270010 Belo Horizonte, MG - Brazil

^[3] Univ Fed Minas Gerais, Inst Ciencias Biol, Dept Parasitol, BR-31270901 Belo Horizonte, MG - Brazil

^[4] Univ Sao Paulo, Fac Med Vet & Zootecnia, Dept Clin Med, Vet Clin Immunol Res Grp, BR-05508270 Sao Paulo - Brazil

^[5] Univ Fed Paraiba, Programa Posgrad Ciencia Anim, BR-58397000 Areia - Brazil

^[6] Univ Sao Paulo, Inst Med Trop, Lab Sorol & Imunobiol, BR-05403000 Sao Paulo - Brazil

^[7] Univ Nacl Toribio Rodriguez de Mendoza Amazonas, Fac Ciencias Salud, Dept Salud Publ, Chachapoyas 01000 - Peru

^[8] Univ Sao Paulo, Inst Ciencias Biomed, Dept Imunol, BR-05508900 Sao Paulo - Brazil

^[9] EMBRAPA Gado Leite, Ave Eugenio do Nascimento 610, BR-36038330 Juiz De Fora - Brazil

^[10] Univ Fed Lavras, Dept Med Vet, BR-37200000 Lavras - Brazil

^[11] Univ Sao Paulo, Fac Med Vet & Zootecnia, Dept Med Vet Prevent & Saude Anim, BR-05508270 Sao Paulo - Brazil

• Document type: Journal article
• Source: JOURNAL OF DAIRY SCIENCE; v. 103, n. 5, p. 4588-4605, MAY 2020.
• Web of Science Citations: 0

Abstract

Staphylococcus aureus is one of the pathogens most frequently isolated from cases of mastitis worldwide. To decrease the effect of S. aureus mastitis in dairy farming, alternative strategies for controlling mastitis are needed that depend on a better knowledge of cowto-cow variations in S. aureus antibody production. The present study sought to explore the diversity of S. aureus antibodies produced by dairy cows with a distinct mastitis history and vaccinated with a polyvalent mastitis vaccine. We obtained protein extracts from S. aureus isolates derived from persistent subclinical mastitis. Proteins were fractionated using 2-dimensional gel electrophoresis and Western blotting. Then, Western blotting membranes were exposed to sera from 24 dairy cows that had been divided into the following groups: vaccinated dairy cows that were infected with S. aureus, further subdivided according to whether they (a) remained infected by S. aureus or (b) recovered from the intramammary infection; unvaccinated dairy cows infected with S. aureus; and vaccinated healthy dairy cows with no history of S. aureus mastitis. Proteins found to be reactive by Western blot were identified by mass spectrometry (MALDI/TOF-TOF). Our most important finding was that F0F1 ATP synthase subunit a, succinyl-diaminopimelate desuccinylase, and cysteinyl-tRNA synthetase were potential candidate proteins for the prevention of S. aureus mastitis. This study strengthens the notion that variations among animals should not be ignored and shows that the heterogeneity of antibody production against anti-staphylococcal antigens in animals may enable the identification of new immunotherapy targets. (AU)

• FAPESP's process: 14/23189-4 - Intramammary infections by Staphylococcus aureus: crosstalk between mammary epithelial cells and peripheral blood mononuclear cells, and how it governs immunity in the mammary gland of dairy cows
• Grantee: Fernando Nogueira de Souza
• Support Opportunities: Scholarships in Brazil - Post-Doctoral