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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Yangambin and Epi-yangambin Isomers: New Purification Method from Ocotea fasciculata and First Cytotoxic Aspects Focusing on In Vivo Safety

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Author(s):
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Martins, Julia [1] ; Coelho, Joana [2] ; Tadini, Maraine Catarina [3, 2] ; de Souza, Rebeca Oliveira [2] ; Figueiredo, Sonia Aparecida [1] ; Vieira Fonseca, Maria Jose [1] ; Perez de Albuquerque, Nayara Cristina [3] ; Lopes, Norberto Peporine [4] ; Berretta, Andresa [2] ; Marquele-Oliveira, Franciane [5] ; Moraes de Oliveira, Anderson Rodrigo [3]
Total Authors: 11
Affiliation:
[1] Univ Sao Paulo, Dept Ciencias Farmaceut, Fac Ciencias Farmaceut Ribeirao Preto, Ribeirao Preto, SP - Brazil
[2] Apis Flora Indl Coml Ltda, Lab Res Dev & Innovat, Ribeirao Preto, SP - Brazil
[3] Univ Sao Paulo, Dept Quim, Fac Filosofia Ciencias & Letras Ribeirao Preto, Ave Bandeirantes 3900, BR-14040901 Ribeirao Preto, SP - Brazil
[4] Univ Sao Paulo, NPPNS, Fac Ciencias Farmaceut Ribeirao Preto, Ribeirao Preto, SP - Brazil
[5] ELEVE Sci, Lab Res Dev & Innovat, Ribeirao Preto, SP - Brazil
Total Affiliations: 5
Document type: Journal article
Source: Planta Medica; v. 86, n. 6, p. 415-424, APR 2020.
Web of Science Citations: 0
Abstract

Ocotea fasciculata presents yangambin (YAN) and its isomer epi-yangambin (EPI-YAN) as major lignans, which are employed as the plant markers for quality control purposes and as potential pharmacological compounds. However, a gap between the pure isomers and safety and efficacy protocols is faced by the scientific community. In this context, this work aimed to report (i) a new and advantageous purifying process in a semi-preparative scale for YAN and EPI-YAN isolation from Ocotea fasciculata , and (ii) an in vitro cytotoxicity study to estimate, for the first time, the LD (50) values of the isolated epimers, as well as the influence of albumin concentration in cell culture medium. The best condition for epimers isolation was achieved in normal-phase liquid chromatography. The lignan fraction (LF), previously obtained from the plant ethanolic extract, was purified yielding 17% and 29% of YAN and EPI-YAN, respectively. The in vitro study demonstrated that YAN and EPI-YAN were safe, and only at the highest concentration studied, a decrease on cell viability was observed. The estimated LD (50) value was higher than 1612 mg/kg for both epimers. The LF, on the other hand, demonstrated an estimated LD (50) of 422 mg/kg. Lignan cytotoxicity studies also evidenced that the higher cell viability was related to the higher concentration of fetal bovine serum as a source of albumin in medium. This is the first time the LD (50) and safety of the isolated epimers were estimated, opening up great perspectives of success in in vivo studies. (AU)

FAPESP's process: 16/07597-0 - Development of chromatographic/electrophoretic methods to be further used in in vitro enzymatic inhibition and drug interaction of chiral pesticides
Grantee:Anderson Rodrigo Moraes de Oliveira
Support type: Regular Research Grants
FAPESP's process: 18/07534-4 - Development of chromatographic / electrophoretic methods to be further applied in in vitro enzymatic inhibition studies and prediction of drug interactions of chiral pesticides - Phase 2
Grantee:Anderson Rodrigo Moraes de Oliveira
Support type: Regular Research Grants