Hypertonic saline reduces cell infiltration into the lungs after brain death in rats

Author(s)

Correia, Cristiano de Jesus ^[1] ; Coutinho e Silva, Raphael dos Santos ^[1] ; Ferreira Soares, Rafaela Garcia ^[1] ; Armstrong Jr, Roberto ; Ricardo-da-Silva, Fernanda Yamamoto ^[2] ; Sannomiya, Paulina ^[2] ; Breithaupt-Faloppa, Ana Cristina ^[2] ; Moreira, Luiz Felipe P. ^[2]

Affiliation

^[1] Univ Sao Paulo, Hosp Clin HCFMUSP, Lab Cirurg Pesquisa Cardiovasc LIM 11, Inst Coracao Incor, Fac Med, Sao Paulo, SP - Brazil

^[2] Armstrong Jr, Jr., Roberto, Univ Sao Paulo, Hosp Clin HCFMUSP, Lab Cirurg Pesquisa Cardiovasc LIM 11, Inst Coracao Incor, Fac Med, Sao Paulo, SP - Brazil

• Document type: Journal article
• Source: Pulmonary Pharmacology & Therapeutics; v. 61, APR 2020.
• Web of Science Citations: 0

Abstract

Background: Lung transplantation is a treatment method for end stage lung disease, but the availability of donor lungs remains a major constraint. Brain death (BD) induces hemodynamic instability with microcirculatory hypoperfusion and increased inflammation, leading to pulmonary dysfunction. Hypertonic saline solution (HSS) is a volume expander possessing immunomodulatory effects. This study evaluated the influence of HSS on pulmonary dysfunction and inflammation in a rat model of BD. Methods: BD was induced by inflation of an intracranial balloon catheter. Rats were divided into {[}1]: Sham, without BD {[}2]; NS, NaCl treatment (0.9%, 4 mL/kg, i.v.) immediately after BD {[}3];HSS1, HSS treatment (NaCl 7.5%, 4 mL/kg, i.v.) immediately after BD; and {[}4] HSS60, HSS treatment 60 min post BD. All groups were analyzed after 360 min. Results: Animals subjected to BD exhibited increased exhaled O-2 and decreased CO2.The number of leukocytes in the lungs was significantly increased in the NS group (p = 0.002) and the HSS treatment was able to reduce it (p = 0.018 and HSS60 = 0.030). In parallel, HSS-treated rats showed reduced levels of ICAM-1 expression, which was increased in the NS compared to Sham group. Lung edema was found increased in the NS group animals compared to Sham and no effect of the HSS treatment was observed. There were no differences among the groups in terms of TNF-alpha, VEGF, and CINC-1 lung concentrations. Conclusions: HSS is capable of reducing inflammatory cell infiltration into the lung after BD induction, which is associated with the reduction of ICAM-1 expression in organ vessels. (AU)

• FAPESP's process: 12/19841-2 - Study of the effects of hypertonic saline solution on microcirculatory and cardiac tissue changes, and development of the inflammatory process in a rat model of brain death
• Grantee: Luiz Felipe Pinho Moreira
• Support Opportunities: Regular Research Grants