| Full text | |
| Author(s): |
Total Authors: 3
|
| Affiliation: | [1] Universidade Federal de São Paulo. Departamento de Biofísica - Brasil
[2] Universidade Federal de São Paulo. Departamento de Biofísica - Brasil
[3] Universidade Federal de São Paulo. Departamento de Biofísica - Brasil
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | Arquivos Brasileiros de Cardiologia; v. 114, n. 4, p. 732-735, 2020-05-29. |
| Abstract | |
Abstract Ranolazine (RANO) prevents cardiac arrhythmia by blocking the late sodium current (INaL). A transmural gradient of Nav1.5 is found in the left ventricular wall of the heart. Thus, we investigated the effects of RANO in healthy cardiomyocytes and in a cellular model of type 3 long QT syndrome (LQT3). We used isolated endocardium (ENDO) and epicardium (EPI) cells and a video edge detection system and fluorescence microscopy to monitor calcium transients. RANO (0.1, 1, 10 and 30 uM, at 25oC) at a range of pacing frequencies showed a minor impact on both cell types, but RANO at 30uM and 35oC for ENDO cells attenuated sarcomere shortening by~21%. Next, to mimic LQT3, we exposed ENDO and EPI cells to anemone toxin II (ATX-II), which augments INaL. Cellular arrhythmias induced by ATX-II were abrogated by RANO (30 µM) at 35oC. Based on our results we can conclude that RANO has a minor impact on sarcomere shortening of healthy ENDO and EPI cells and it abrogates arrhythmias induced by INaLto a similar level in ENDO and EPI cells. (AU) | |
| FAPESP's process: | 14/09861-1 - The role of late sodium current in the inherited and acquired cardiac arrhythmias: from the biophysics properties to new therapeutic targets |
| Grantee: | Danilo Roman Campos |
| Support Opportunities: | Research Grants - Young Investigators Grants |