Epigallocatechin Gallate Enhances MAL-PDT Cytotoxic Effect on PDT-Resistant Skin Cancer Squamous Cells

Author(s):Show less -

Leon, Daniela ^[1] ; Buchegger, Kurt ^{[1, 2]} ; Silva, Ramon ^[3] ; Riquelme, Ismael ^[3] ; Viscarra, Tamara ^[1] ; Mora-Lagos, Barbara ^[1] ; Zanella, Louise ^[1] ; Schafer, Fabiola ^[4] ; Kurachi, Cristina ^[5] ; Roa, Juan Carlos ^[6] ; Ili, Carmen ^[1] ; Brebi, Priscilla ^[1]

Affiliation

^[1] Univ La Frontera, Lab Integrat Biol, Ctr Excelencia Med Traslac Sci & Technol Bioresoc, Temuco 4810296 - Chile

^[2] Univ La Frontera, Sch Med, Dept Basic Sci, Temuco 4811230 - Chile

^[3] Univ Autonoma Chile, Fac Ciencias Salud, Inst Ciencias Biomed, Temuco 4810101 - Chile

^[4] Univ La Frontera, Sch Med, Dept Med Specialties, Temuco 4811230 - Chile

^[5] Univ Sao Paulo, Sao Carlos Inst Phys, POB 369, BR-13560970 Sao Carlos, SP - Brazil

^[6] Pontificia Univ Catolica Chile, Dept Pathol, Santiago 8330024 - Chile

• Document type: Journal article
• Source: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES; v. 21, n. 9 MAY 2020.
• Web of Science Citations: 0

Abstract

Photodynamic therapy (PDT) has been used to treat certain types of non-melanoma skin cancer with promising results. However, some skin lesions have not fully responded to this treatment, suggesting a potential PDT-resistant phenotype. Therefore, novel therapeutic alternatives must be identified that improve PDT in resistant skin cancer. In this study, we analyzed the cell viability, intracellular protoporphyrin IX (PpIX) content and subcellular localization, proliferation profile, cell death, reactive oxygen species (ROS) detection and relative gene expression in PDT-resistant HSC-1 cells. PDT-resistant HSC-1 cells show a low quantity of protoporphyrin IX and low levels of ROS, and thus a low rate of death cell. Furthermore, the resistant phenotype showed a downregulation of HSPB1, SLC15A2, FECH, SOD2 and an upregulation of HMBS and BIRC5 genes. On the other hand, epigallocatechin gallate catechin enhanced the MAL-PDT effect, increasing levels of protoporphyrin IX and ROS, and killing 100% of resistant cells. The resistant MAL-PDT model of skin cancer squamous cells (HSC-1) is a reliable and useful tool to understand PDT cytotoxicity and cellular response. These resistant cells were successfully sensitized with epigallocatechin gallate catechin. The in vitro epigallocatechin gallate catechin effect as an enhancer of MAL-PDT in resistant cells is promising in the treatment of difficult skin cancer lesions. (AU)

• FAPESP's process: 13/07276-1 - CEPOF - Optics and Photonic Research Center
• Grantee: Vanderlei Salvador Bagnato
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC