| Full text | |
| Author(s): |
Castillo Pedraza, Midian C.
[1, 2]
;
de Oliveira Fratucelli, Erick Dante
[1]
;
Ribeiro, Sabrina Marcela
[1]
;
Florez Salamanca, Elkin Jahir
[1]
;
Colin, Jaqueline Silva
[1]
;
Klein, I, Marlise
Total Authors: 6
|
| Affiliation: | [1] I, Sao Paulo State Univ UNESP, Sch Dent, Dept Dent Mat & Prosthodont, Rua Humaita 1680, BR-14801903 Araraquara, SP - Brazil
[2] Univ Magdalena, Fac Hlth Sci, Santa Marta 470003 - Colombia
Total Affiliations: 2
|
| Document type: | Journal article |
| Source: | Molecules; v. 25, n. 9 MAY 2020. |
| Web of Science Citations: | 0 |
| Abstract | |
Dental caries is a diet-biofilm-dependent disease. Streptococcus mutans contributes to cariogenic biofilms by producing an extracellular matrix rich in exopolysaccharides and acids. The study aimed to determine the effect of topical treatments with compound 1771 (modulates lipoteichoic acid (LTA) metabolism) and myricetin (affects the synthesis of exopolysaccharides) on S. mutans biofilms. In vitro S. mutans UA159 biofilms were grown on saliva-coated hydroxyapatite discs, alternating 0.1% sucrose and 0.5% sucrose plus 1% starch. Twice-daily topical treatments were performed with both agents alone and combined with and without fluoride: compound 1771 (2.6 mu g/mL), myricetin (500 mu g/mL), 1771 + myricetin, fluoride (250 ppm), 1771 + fluoride, myricetin + fluoride, 1771 + myricetin + fluoride, and vehicle. Biofilms were evaluated via microbiological, biochemical, imaging, and gene expression methods. Compound 1771 alone yielded less viable counts, biomass, exopolysaccharides, and extracellular LTA. Moreover, the combination 1771 + myricetin + fluoride decreased three logs of bacterium counts, 60% biomass, >74% exopolysaccharides, and 20% LTA. The effect of treatments on extracellular DNA was not pronounced. The combination strategy affected the size of microcolonies and exopolysaccharides distribution and inhibited the expression of genes linked to insoluble exopolysaccharides synthesis. Therefore, compound 1771 prevented the accumulation of S. mutans biofilm; however, the effect was more pronounced when it was associated with fluoride and myricetin. (AU) | |
| FAPESP's process: | 17/07408-6 - Evaluation of antimicrobial and antibiofilm activities of extracts and fractions from plants against oral pathogenic microorganisms |
| Grantee: | Sabrina Marcela Ribeiro |
| Support Opportunities: | Scholarships in Brazil - Master |
| FAPESP's process: | 14/05423-0 - Role of extracellular DNA and lipoteichoic acid in the matrix of cariogenic biofilm |
| Grantee: | Marlise Inêz Klein Furlan |
| Support Opportunities: | Research Grants - Young Investigators Grants |
| FAPESP's process: | 17/26623-5 - Influence of Streptococcus mutans on the matrix assembly during the development of microcosm biofilms exposed to nutritional challenge |
| Grantee: | Elkin Jahir Florez Salamanca |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 17/00753-0 - In vivo virulence analyses of Streptococcus mutans lytTS genes and operon dltABCD and modulation thereof by targeted therapy |
| Grantee: | Midian Clara Castillo Pedraza |
| Support Opportunities: | Scholarships in Brazil - Doctorate |