| Full text | |
| Author(s): |
Buranello, Patricia A. A.
[1, 2]
;
Barbosa-Lorenzi, Valeria C.
[2]
;
Pinto, Marcelo R.
[3, 2]
;
Pereira-da-Silva, Gabriela
[4]
;
Barreira, Maria Cristina R. A.
[2]
;
Jamur, Maria Celia
[2]
;
Oliver, Constance
[2]
Total Authors: 7
|
| Affiliation: | [1] Univ Fed Triangulo Mineiro, Dept Biol Sci, Uberaba, MG - Brazil
[2] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Cell & Mol Biol & Pathogen Bioagents, Ribeirao Preto, SP - Brazil
[3] Univ Uberaba, Lab Biopathol & Mol Biol, Uberaba, MG - Brazil
[4] Univ Sao Paulo, Dept Maternal Infant Nursing & Publ Hlth, Escola Enfermagem Ribeirao Preto, Ribeirao Preto, SP - Brazil
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | PLoS One; v. 15, n. 3 MAR 24 2020. |
| Web of Science Citations: | 0 |
| Abstract | |
Mast cells are connective tissue resident cells with morphological and functional characteristics that contribute to their role in allergic and inflammatory processes, host defense and maintenance of tissue homeostasis. Mast cell activation results in the release of pro-inflammatory mediators which are largely responsible for the physiological functions of mast cells. The lectin ArtinM, extracted from Artocarpus heterophyllus (jackfruit), binds to D-manose, thus inducing degranulation of mast cells. ArtinM has several immunomodulatory properties including acceleration of wound healing, and induction of cytokine release. The aim of the present study was to investigate the role of ArtinM in the activation and proliferation of mast cells. The rat mast cell line RBL-2H3 was used throughout this study. At a low concentration (0.25 mu g/mL), ArtinM induced mast cell activation and the release of IL-6 without stimulating the release of pre-formed or newly formed mediators. Additionally, when the cells were activated by ArtinM protein tyrosine phosphorylation was stimulated. The low concentration of ArtinM also activated the transcription factor NFkB, but not NFAT. ArtinM also affected the cell cycle and stimulated cell proliferation. Therefore, ArtinM may have therapeutic applications by modulating immune responses due to its ability to activate mast cells and promote the release of newly synthesized mediators. Additionally, ArtinM could have beneficial effects at low concentrations without degranulating mast cells and inducing allergic reactions. (AU) | |
| FAPESP's process: | 09/54013-0 - Functional correlation between mast cells and tumor angiogenesis |
| Grantee: | Maria Célia Jamur |
| Support Opportunities: | Multi-user Equipment Program |
| FAPESP's process: | 13/04088-0 - Lectin from pathogens |
| Grantee: | Maria Cristina Roque Antunes Barreira |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 06/60642-2 - Lectins: biological effects and pharmaceutical applications |
| Grantee: | Maria Cristina Roque Antunes Barreira |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 17/18618-1 - Interaction between mast cells and endothelial cells during in vitro angiogenesis |
| Grantee: | Constance Oliver |
| Support Opportunities: | Regular Research Grants |