| Full text | |
| Author(s): |
Romagnoli, Camila Lopes
[1]
;
Machado Pellegrino, Katia Cristina
[2]
;
Silva, Natalia Maria
[1]
;
Brianesi, Urze Adomaitis
[1]
;
Leao, Sylvia Cardoso
[1]
;
da Silva Rabello, Michelle Christiane
[3]
;
Viana-Niero, Cristina
[1]
Total Authors: 7
|
| Affiliation: | [1] Univ Fed Sao Paulo, Dept Microbiol Imunol & Parasitol, Sao Paulo, SP - Brazil
[2] Univ Fed Sao Paulo, Dept Ecol & Biol Evolut, Diadema, SP - Brazil
[3] Fundacao Oswaldo Cruz, Inst Aggeu Magalhaes, Dept Imunol, Recife, PE - Brazil
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | PLoS One; v. 15, n. 1 JAN 14 2020. |
| Web of Science Citations: | 0 |
| Abstract | |
We investigated the species diversity of Mycobacteriaceae in surface water samples from six environments at the zoological park in Sao Paulo, Brazil. Three hundred and eighty isolates were cultivated and identified by phenotypic characteristics (growth rate and pigmentation) and sequencing of hsp65, rpoB and 16S rRNA genes. The results revealed that almost 48% of the isolates could be identified at the species level; about 50% were classified at the genus level, and only less than 2% of the isolates showed an inconclusive identification. The isolates classified at the genus level and not identified were then evaluated by phylogenetic analyses using the same three concatenated target genes. The results allowed us to identify at the genus level some isolates that previously had inconclusive identification, and they also suggested the presence of putative candidate species within the sample, demonstrating that this zoological park is an important source of diversity. (AU) | |
| FAPESP's process: | 11/13140-0 - Isolation and identification of mycobacteria originating from untreated and treated water from Fundação Parque Zoológico de São Paulo |
| Grantee: | Urze Adomaitis Brianesi |
| Support Opportunities: | Scholarships in Brazil - Master |