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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Atco, a yeast mitochondrial complex of Atp9 and Cox6, is an assembly intermediate of the ATP synthase

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Author(s):
Ribeiro Franco, Leticia Veloso [1, 2] ; Su, Chen-Hsien [1] ; Burnett, Julia [1] ; Teixeira, Lorisa Simas [1] ; Tzagoloff, Alexander [1]
Total Authors: 5
Affiliation:
[1] Columbia Univ, Dept Biol Sci, New York, NY 10027 - USA
[2] Univ Sao Paulo, Dept Microbiol, Sao Paulo, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: PLoS One; v. 15, n. 5 MAY 15 2020.
Web of Science Citations: 0
Abstract

Mitochondrial oxidative phosphorylation (oxphos) is the process by which the ATP synthase conserves the energy released during the oxidation of different nutrients as ATP. The yeast ATP synthase consists of three assembly modules, one of which is a ring consisting of 10 copies of the Atp9 subunit. We previously reported the existence in yeast mitochondria of high molecular weight complexes composed of mitochondrially encoded Atp9 and of Cox6, an imported structural subunit of cytochrome oxidase (COX). Pulse-chase experiments indicated a correlation between the loss of newly translated Atp9 complexed to Cox6 and an increase of newly formed Atp9 ring, but did not exclude the possibility of an alternate source of Atp9 for ring formation. Here we have extended studies on the functions and structure of this complex, referred to as Atco. We show that Atco is the exclusive source of Atp9 for the ATP synthase assembly. Pulse-chase experiments show that newly translated Atp9, present in Atco, is converted to a ring, which is incorporated into the ATP synthase with kinetics characteristic of a precursor-product relationship. Even though Atco does not contain the ring form of Atp9, cross-linking experiments indicate that it is oligomeric and that the intersubunit interactions are similar to those of the bona fide ring. We propose that, by providing Atp9 for biogenesis of ATP synthase, Atco complexes free Cox6 for assembly of COX. This suggests that Atco complexes may play a role in coordinating assembly and maintaining proper stoichiometry of the two oxphos enzymes (AU)

FAPESP's process: 19/02799-2 - ATCO1, a mitochondrial master regulator of ATP synthase and cytochrome oxidase biogenesis?
Grantee:Leticia Veloso Ribeiro Franco
Support Opportunities: Scholarships in Brazil - Post-Doctoral