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Study of the genotoxic effect, the membrane potential and cell death pathways of curcumin in photodynamic therapy in promastigote and amastigote forms of L. major and L. brasiliensis: in vitro


The Leshmaniasis are infectious diseases caused by protozoa of the genus Leishmania that have two evolutionary forms: one flagellated form or promastigote, responsible for the infection and a unflagellated form or the amastigote form of the parasite that multiplies in the vertebrate host. The disease can manifest itself in cutaneous forms, mucocutaneous or visceral, the last being the most severe form of the disease. The cutaneous leishmaniasis is present in 80 countries, it is considered by the World Health Organization a public health problem, with an estimated 65,000 new cases per year in Brazil, the country with the highest prevalence of the disease in the Americas. The conventional treatment is performed with pentavalent antimonials, Pentamidinas and antibiotics, but they are aggressive treatments to patients and cause side effects such as nausea, vomiting and myalgia. Photodynamic therapy combines a photosensitive substance, a light source and molecular oxygen to induce the formation of reactive oxygen species leading to impracticability of target cells by apoptosis. It is an attractive alternative for the treatment of cutaneous form the selectivity of the treatment field, making it possible to treat individual lesions, without the impact of systemically administered drugs have. The objective of this project is to evaluate the genotoxic effect of PDT with curcumin in promastigotes and amastigote Leishmania major and Leishmania braziliensis through the comet assay, verify the presence of apoptosis and necrosis induced by PDT, the assay with Annexin V and iodide propidium and evaluate changes in mitochondrial membrane potential using rhodamine trimethylated or 123, and verify the kinetics of internalization of curcumin with confocal microscopy and evaluate the expression of mitochondrial genes LBRM_30_3630 (putative ATP synthase, epsilon chain), LMJF_25_1180 (putative ATPase beta subunit) and F0F1-ATP synthase (Complex V) by quantitative PCR and real time. (AU)

Scientific publications (5)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
PINTO, JULIANA G.; MARCOLINO, LUCIANA M. C.; FERREIRA-STRIXINO, JULIANA. Photodynamic activity of Photogem(R) in Leishmania promastigotes and infected macrophages. FUTURE MICROBIOLOGY, v. 16, n. 2 JAN 2021. Web of Science Citations: 0.
SAKANE, KUMIKO KOIBUCHI; BHATTACHARJEE, TANMOY; FAGUNDES, JACIARA; MARCOLINO, LUCIANA MARIA CORTEZ; FERREIRA, ISABELLE; PINTO, JULIANA GUERRA; FERREIRA-STRIXINO, JULIANA. Biochemical changes in Leishmania braziliensis after photodynamic therapy with methylene blue assessed by the Fourier transform infrared spectroscopy. Lasers in Medical Science, AUG 2020. Web of Science Citations: 0.
FERREIRA-STRIXINO, JULIANA. Comparison of photodynamic therapy with methylene blue associated with ceftriaxone in gram-negative bacteria; an in vitro study. Photodiagnosis and Photodynamic Therapy, v. 30, JUN 2020. Web of Science Citations: 0.
FERREIRA-STRIXINO, JULIANA. Analysis of the effect of photodynamic therapy with Fotoenticine on gliosarcoma cells. Photodiagnosis and Photodynamic Therapy, v. 30, JUN 2020. Web of Science Citations: 0.
FREITAS, MIRIAN A. A.; PEREIRA, ANDRE H. C.; PINTO, JULIANA G.; CASAS, ADRIANA; FERREIRA-STRIXINO, JULIANA. Bacterial viability after antimicrobial photodynamic therapy with curcumin on multiresistant Staphylococcus aureus. FUTURE MICROBIOLOGY, v. 14, n. 9, p. 739-748, JUL 4 2019. Web of Science Citations: 3.

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