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Study of the genotoxic effect, the membrane potential and cell death pathways of curcumin in photodynamic therapy in promastigote and amastigote forms of L. major and L. braziliensis: in vitro

Grant number: 16/12211-4
Support Opportunities:Regular Research Grants
Duration: December 01, 2016 - November 30, 2018
Field of knowledge:Biological Sciences - Biophysics - Radiology and Photobiology
Principal Investigator:Juliana Ferreira Strixino
Grantee:Juliana Ferreira Strixino
Host Institution: Instituto de Pesquisa e Desenvolvimento (IP&D). Universidade do Vale do Paraíba (UNIVAP). São José dos Campos , SP, Brazil
Associated researchers:Cristina Pacheco Soares ; Isabelle Ferreira ; Juliana Guerra Pinto ; Renata de Azevedo Canevari


The Leshmaniasis are infectious diseases caused by protozoa of the genus Leishmania that have two evolutionary forms: one flagellated form or promastigote, responsible for the infection and a unflagellated form or the amastigote form of the parasite that multiplies in the vertebrate host. The disease can manifest itself in cutaneous forms, mucocutaneous or visceral, the last being the most severe form of the disease. The cutaneous leishmaniasis is present in 80 countries, it is considered by the World Health Organization a public health problem, with an estimated 65,000 new cases per year in Brazil, the country with the highest prevalence of the disease in the Americas. The conventional treatment is performed with pentavalent antimonials, Pentamidinas and antibiotics, but they are aggressive treatments to patients and cause side effects such as nausea, vomiting and myalgia. Photodynamic therapy combines a photosensitive substance, a light source and molecular oxygen to induce the formation of reactive oxygen species leading to impracticability of target cells by apoptosis. It is an attractive alternative for the treatment of cutaneous form the selectivity of the treatment field, making it possible to treat individual lesions, without the impact of systemically administered drugs have. The objective of this project is to evaluate the genotoxic effect of PDT with curcumin in promastigotes and amastigote Leishmania major and Leishmania braziliensis through the comet assay, verify the presence of apoptosis and necrosis induced by PDT, the assay with Annexin V and iodide propidium and evaluate changes in mitochondrial membrane potential using rhodamine trimethylated or 123, and verify the kinetics of internalization of curcumin with confocal microscopy and evaluate the expression of mitochondrial genes LBRM_30_3630 (putative ATP synthase, epsilon chain), LMJF_25_1180 (putative ATPase beta subunit) and F0F1-ATP synthase (Complex V) by quantitative PCR and real time. (AU)

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Scientific publications (8)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
SAKANE, KUMIKO KOIBUCHI; BHATTACHARJEE, TANMOY; FAGUNDES, JACIARA; MARCOLINO, LUCIANA MARIA CORTEZ; FERREIRA, ISABELLE; PINTO, JULIANA GUERRA; FERREIRA-STRIXINO, JULIANA. Biochemical changes in Leishmania braziliensis after photodynamic therapy with methylene blue assessed by the Fourier transform infrared spectroscopy. Lasers in Medical Science, v. 36, n. 4, . (16/12211-4)
VITORIO, GABRIELLE DOS SANTOS; DE ALMEIDA, RAINARA MORENO SANCHES; PINTO, JULIANA GUERRA; FONTANA, LETICIA CORREA; FERREIRA-STRIXINO, JULIANA. Analysis of the effects of Photodynamic therapy with Photodithazine on the treatment of 9l/lacZ cells, in vitro study. Photodiagnosis and Photodynamic Therapy, v. 34, . (18/12274-1, 13/07276-1, 16/12211-4, 18/15302-6)
PEREIRA, ANDRE HENRIQUE CORREIA; MARCOLINO, LUCIANA MARIA CORTEZ; PINTO, JULIANA GUERRA; FERREIRA-STRIXINO, JULIANA. Evaluation of the Photodynamic Therapy with Curcumin on L. braziliensis and L. major Amastigotes. ANTIBIOTICS-BASEL, v. 10, n. 6, . (16/12211-4, 13/07276-1)
PINTO, JULIANA G.; MARCOLINO, LUCIANA M. C.; FERREIRA-STRIXINO, JULIANA. Photodynamic activity of Photogem(R) in Leishmania promastigotes and infected macrophages. FUTURE MICROBIOLOGY, v. 16, n. 2, . (13/07276-1, 13/12284-3, 16/12211-4)
FERREIRA-STRIXINO, JULIANA. Analysis of the effect of photodynamic therapy with Fotoenticine on gliosarcoma cells. Photodiagnosis and Photodynamic Therapy, v. 30, . (16/20022-7, 17/01469-3, 13/07276-1, 16/12211-4)
RIBEIRO, ISABELLE DE PAULA; PINTO, JULIANA GUERRA; NUNES SOUZA, BEATRIZ MULLER; GUILLERMO MINAN, ALEJANDRO; FERREIRA-STRIXINO, JULIANA. ntimicrobial photodynamic therapy with curcumin on methicillin-resistant Staphylococcus aureus biofil. Photodiagnosis and Photodynamic Therapy, v. 37, . (13/07276-1, 16/12211-4)
FREITAS, MIRIAN A. A.; PEREIRA, ANDRE H. C.; PINTO, JULIANA G.; CASAS, ADRIANA; FERREIRA-STRIXINO, JULIANA. Bacterial viability after antimicrobial photodynamic therapy with curcumin on multiresistant Staphylococcus aureus. FUTURE MICROBIOLOGY, v. 14, n. 9, p. 739-748, . (16/12211-4)

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