Evaluation of antileishmanial activity of antihistaminic drugs and identification of cellular alteration related to therapeutic response in Leishmania (Leishmania) infantum

• Grant number: 14/24908-4
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): April 01, 2015
• Effective date (End): December 31, 2015
• Field of knowledge: Biological Sciences - Parasitology - Protozoology of Parasites
• Principal Investigator: Samanta Etel Treiger Borborema de Carvalho
• Grantee: Viviane de Melo Mendes
• Host Institution: Instituto Adolfo Lutz (IAL). Coordenadoria de Controle de Doenças (CCD). Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil

Abstract

The Leishmaniases are a complex of antropozoonotic infectious diseases caused by protozoan parasites Leishmania and transmitted by sandflies. Leishmaniasis is considered a major public health problem and represents a group of diseases with significant clinical and epidemiological diversity spectrum. Therapeutic regimen is restricted, due to toxicity, poor efficacy, resistance, cost and inconvenience of the treatment regimens there is an urgent need to identify and develop new therapies. Repositioning of drugs is a therapeutic approach that has shown satisfactory results in the treatment of Leishmaniasis. Besides the identification of new drugs, characterization of the mechanism and metabolic pathway involved in the therapeutic response is a useful and effective strategy to improve therapy. This study aims to evaluate the antileishmanial activity of antihistaminic drugs and identify cellular alteration related to therapeutic response. For this, the in vitro effectiveness of drugs in Leishmania infantum promastigotes and L. infantum-infected peritoneal macrophages will be evaluated and cytotoxicity in mammalian cells will be assessed. Cellular changes in the plasma membrane and in the mitochondria in promastigote form will be analyzed by spectrofluorimetry. This work, in addition to presenting direct results for the therapy of Leishmaniasis, with the development of new alternatives, has great potential as an assessment of therapeutic response of various types of drugs. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

TREIGER BORBOREMA, SAMANTA ETEL; OSSO JUNIOR, JOAO ALBERTO; TEMPONE, ANDRE GUSTAVO; DE ANDRADE JUNIOR, HEITOR FRANCO; DO NASCIMENTO, NANCI. Pharmacokinetic of meglumine antimoniate encapsulated in phosphatidylserine-liposomes in mice model: A candidate formulation for visceral leishmaniasis. BIOMEDICINE & PHARMACOTHERAPY, v. 103, p. 1609-1616, JUL 2018. (14/24908-4)

MENDES, VIVIANE DE MELO; TEMPONE, ANDRE GUSTAVO; TREIGER BORBOREMA, SAMANTA ETEL. Antileishmanial activity of Hi-antihistamine drugs and cellular alterations in Leishmania (L.) infantum. Acta Tropica, v. 195, p. 6-14, JUL 2019. (18/10279-6, 14/24908-4)