High occurrence of beta-lactamase-producing Salmonella Heidelberg from poultry origin

Author(s)

Souza, Andrei I. S. ^{[1, 2]} ; Saraiva, Mauro M. S. ^[1] ; Casas, Monique R. T. ^[3] ; Oliveira, Gustavo M. ^[1] ; Cardozo, V, Marita ; Benevides, Valdinete P. ^{[1, 2]} ; Barbosa, Fernanda O. ^{[1, 4]} ; Freitas Neto, Oliveiro C. ^[5] ; Almeida, Adriana M. ^[1] ; Berchieri Junior, Angelo ^[1]

Affiliation

^[1] Sao Paulo State Univ Unesp, Sch Agr & Veterinarian Sci, Dept Vet Pathol, Lab Avian Pathol, Jaboticabal, SP - Brazil

^[2] Sao Paulo State Univ Unesp, Sch Agr & Veterinarian Sci, Agr & Livestock Microbiol Postgrad Program, Jaboticabal, SP - Brazil

^[3] Adolfo Lutz Inst, Nucleus Enter Dis & Infect Special Pathogens Ctr, Sao Paulo, SP - Brazil

^[4] Sao Paulo State Univ Unesp, Sch Agr Ad Veterinarian Sci, Vet Med Postgrad Program, Jaboticabal, SP - Brazil

^[5] Fed Univ Minas Gerais UFMG, Belo Horizonte, MG - Brazil

• Document type: Journal article
• Source: PLoS One; v. 15, n. 3 MAR 31 2020.
• Web of Science Citations: 1

Abstract

Salmonella Heidelberg is commonly reported in foodborne outbreaks around the world, and chickens and poultry products are known as important source of these pathogen. Multidrug-resistant S. Heidelberg strains are disseminated into poultry production chair, which can lead to severe clinical infections in humans and of difficult to treat. This study aimed at evaluating the beta-lactam susceptibility and genotypic relatedness of Salmonella Heidelberg at Brazilian poultry production chain. Sixty-two S. Heidelberg strains from poultry production chain (poultry, poultry meat and poultry farm) were used. All strains were evaluated to antimicrobial susceptibility by diffusion disk test, as well as beta-lactam resistance genes. Genotypic relatedness was assessed by Pulsed-Field Gel Eletrophoresis, using Xba1 restriction enzyme. Forty-one strains were characterized as multidrug-resistant according to phenotype characterization. The resistance susceptibility revealed 31 distinct profiles, with higher prevalence of streptomycin (61/62), nalidixic acid (50/62), tetracycline (43/62) and beta-lactam drugs (37/62). bla(CMY-2) was the more frequent beta-lactamase gene found (38/62); other resistance genes found were bla(CTX-M) (2/62), bla(SHV) (3/62) and bla(TEM-1) (38/62). No carbapenemase genes was found. The Pulsed-Field Gel Electrophoresis showed 58 different profiles. Strains with a larger number of antimicrobial resistance were grouped into ten major clusters apart from others. The spread of resistance by ampC continues to rise, thereby turning concern to public health, since the beta-lactam antimicrobials are used as a therapeutic treatment in humans. (AU)

• FAPESP's process: 17/25200-3 - Analysis of genomic and antimicrobial resistance profiles of Salmonella Heidelberg strains
• Grantee: Andrei Itajahy Secundo de Souza
• Support Opportunities: Scholarships in Brazil - Master