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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Differences in gene modulation in Saccharomyces cerevisiae indicate that maturity plays an important role in the high hydrostatic pressure stress response and resistance

Full text
Author(s):
Spagnol, Brigida A. A. [1] ; Antunes, Tathiana F. S. [1] ; Quadros, Oeber F. [1] ; Fernandes, A. Alberto R. [1] ; Fernandes, Patricia M. B. [1]
Total Authors: 5
Affiliation:
[1] Fed Univ Espirito Santo UFES, Biotechnol Core, BR-29040090 Vitoria, ES - Brazil
Total Affiliations: 1
Document type: Journal article
Source: FUNGAL BIOLOGY; v. 124, n. 5, p. 440-446, MAY 2020.
Web of Science Citations: 1
Abstract

There is a strong relationship between the regulatory pathways to oxidative stress, longevity, and aging. High hydrostatic pressure (HHP) induces oxidative stress and activates cellular defense mechanisms. The understanding of these mechanisms is a strategy to delay damage associated with aging. Addressing resistance to stress and aging in Saccharomyces cerevisiae is a well-accepted approach since pathways involved in energy balance, damage accumulation and stress response are preserved among eukaryotes. The purpose of this study was to correlate the environmental stress response to cell maturity. HHP stress response on S. cerevisiae mother and daughter cells was evaluated through survival, reactive oxygen species (ROS) accumulation and gene expression. Mature cells were yeasts that had budded and originated at least one descendant, and young cells were the ones that did not form a bud. Mature cells were more resistant to HHP, although they showed a decrease in expression of antioxidants enzymes genes, and a higher intracellular levels of ROS. Young cells had less resistance to HHP despite a tendency of positively regulating these same antioxidant encoders. The TOR1 gene, related to aging and apoptosis, was unchanged in mother cells and showed a tendency toward increased expression in daughter cells submitted to HHP. The gene modulation differences of the mother and daughter cells indicates that maturity plays an important role in the HHP stress response and resistance. Thus, even accumulating high levels of ROS, mature cells were more tolerant to HHP stress and survived better, despite aging. (C) 2019 British Mycological Society. Published by Elsevier Ltd. All rights reserved. (AU)

FAPESP's process: 18/20571-6 - International Symposium on Fungal Stress - ISFUS
Grantee:Drauzio Eduardo Naretto Rangel
Support Opportunities: Organization Grants - Scientific Meeting