Development of a system to treat and online monitor photodynamic therapy of skin cancer using PpIX near-infrared fluorescence

Author(s)

Garcia, Marlon Rodrigues ^{[1, 2]} ; Requena, Michelle Barreto ^[1] ; Pratavieira, Sebastiao ^[1] ; Moriyama, Lilian Tan ^[1] ; Becker, Marcelo ^{[2, 3]} ; Bagnato, Vanderlei Salvador ^{[1, 4, 5]} ; Kurachi, Cristina ^[1] ; Magalhaes, Daniel Varela ^{[2, 3]}

Affiliation

^[1] Univ Sao Paulo, Sao Carlos Inst Phys, BR-13566590 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Sao Carlos Sch Engn, Dept Mech Engn, BR-13566590 Sao Paulo - Brazil

^[3] Bagnato, Vanderlei Salvador, Texas A\&M Univ, Dept Biomed Engn, College Stn, TX USA.Garcia, Marlon Rodrigues, Univ Sao Paulo, Sao Carlos Sch Engn, Dept Mech Engn, BR-13566590 Sao Paulo - Brazil

^[4] Texas A&M Univ, Hagler Inst Adv Study, College Stn, TX - USA

^[5] Texas A&M Univ, Dept Biomed Engn, College Stn, TX - USA

• Document type: Journal article
• Source: Photodiagnosis and Photodynamic Therapy; v. 30, JUN 2020.
• Web of Science Citations: 0

Abstract

The limited adoption of photodynamic therapy (PDT) around the medical field may be tied to the unpredicted treatment response that an unmonitored therapy could deliver. Given the high variability in the lesions optical and physiological parameters, it is of fundamental importance to monitor PDT, since different lesions require different therapeutic parameters. We developed a system to treat and online monitor PDT of skin cancer, using protoporphyrin-IX (PpIX) near-infrared fluorescence imaging. The system can be operated up to 150 mW/cm(2) at 633 nm, with real-time fluorescence monitoring around 700 nm, using the treatment light itself for fluorescence excitation. This technology allows system portability, simplicity, and low cost. This study describes the system development and its comparison with a 400-450 nm commercial system to detect the PpIX fluorescence during a PDT in murine skin cancer model. The developed device was able to acquire considerably more fluorescence signal from deeper regions when compared to the violet excitation device. (AU)

• FAPESP's process: 09/54035-4 - Facility for advanced studies of biosystems and nanostructured materials
• Grantee: Igor Polikarpov
• Support Opportunities: Multi-user Equipment Program
• FAPESP's process: 13/07276-1 - CEPOF - Optics and Photonic Research Center
• Grantee: Vanderlei Salvador Bagnato
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC
• FAPESP's process: 14/50857-8 - National Institute in Basic Optics and Applied to Life Sciences
• Grantee: Vanderlei Salvador Bagnato
• Support Opportunities: Research Projects - Thematic Grants