Aloe veraand Honey Solution and Their Ethanolic Extraction Solution Could Act on Metastasis-Regulating Processes in Walker 256 Tumor Tissues In Vivo?

Author(s)

Tomasin, Rebeka ^[1] ; Rheder Fagundes Pascoal, Aislan Cristina ^{[2, 3, 4]} ; Salvador, Marcos Jose ^{[2, 3]} ; Cintra Gomes-Marcondes, Maria Cristina ^[1]

Affiliation

^[1] Univ Estadual Campinas, Inst Biol, Dept Struct & Funct Biol, Lab Nutr & Canc, UNICAMP, Campinas Cp6109, BR-13083970 Campinas, SP - Brazil

^[2] Univ Estadual Campinas, Inst Biol, Dept Plant Biol, PPGBTPB, Campinas, SP - Brazil

^[3] Univ Estadual Campinas, Inst Biol, PPGBCE, Campinas, SP - Brazil

^[4] Univ Fed Fluminense UFF, Inst Saude Nova Friburgo, Nova Friburgo, RJ - Brazil

• Document type: Journal article
• Source: NUTRITION AND CANCER-AN INTERNATIONAL JOURNAL; JUN 2020.
• Web of Science Citations: 0

Abstract

Research has shown that bothAloe veraand honey have anticancer and nutrition properties, including the inhibition of metastasis. In order to evaluate the effect of a solution ofAloe veraand honey (A) and their ethanolic fraction (F) on metastasis-regulating processes in primary tumors, Wistar rats were subcutaneously implanted with Walker 256 tumors and treated with A and F (670 mu l/kg by gavage, daily for 21 days). An analysis of the primary tumor tissues of these animals showed a decrease in N-cadherin expression in groups WA and WF, with a concomitant increase in E-cadherin expression in group WA compared to the control group. Cathepsin D activity was also decreased in the tumor tissues from groups WA and WF. In addition, the number of blood vessels and their diameter significantly reduced in tumor tissues from groups WA and WF compared to those from control group. UHPLC-ESI-MS/MS analysis of the samples A and F, suggested presence of molecules with verified antitumor activity, including caffeic acid, ferulic acid, mannose, aloin A, aloin B, pinocembrin, chrysin, and kaempferol. These data showed that treatment with A and F could reduce the metastatic propensity of tumors by modulating neoangiogenesis and the process of epithelial-to-mesenchymal transition. (AU)

• FAPESP's process: 10/00209-9 - Nutritional supplementation and cancer cachexia. Biochemical and molecular studies
• Grantee: Maria Cristina Cintra Gomes Marcondes
• Support type: Regular Research Grants
• FAPESP's process: 17/02739-4 - Nutrition and cancer: study of molecular, proteomic and metabolomic aspects of experimental model of cachexia
• Grantee: Maria Cristina Cintra Gomes Marcondes
• Support type: Research Projects - Thematic Grants
• FAPESP's process: 11/17067-5 - Campomanesia adamantium and Campomanesia guaviroba: Phytochemical Study and Evaluation of Biological Activities in vitro and in vivo.
• Grantee: Aislan Cristina Rheder Fagundes Pascoal
• Support type: Scholarships in Brazil - Doctorate
• FAPESP's process: 14/13334-7 - Nutrition and cancer: metabolomic, and proteomic profile of muscle, placental, fetal, and tumor cell signaling pathways in Walker-256 tumour-bearing pregnant rats submitted to leucine nutritional supplementation
• Grantee: Maria Cristina Cintra Gomes Marcondes
• Support type: Regular Research Grants
• FAPESP's process: 15/03726-8 - Integrated chemical and biological study in the search for bioactive substances in plants* and development of analytical procedures. *(Amaranthaceae, Gesneriaceae, Myrtaceae e Annonaceae)
• Grantee: Marcos José Salvador
• Support type: Regular Research Grants
• FAPESP's process: 10/00714-5 - "Modulatory Effects of Aloe vera and honey in tumour growth"
• Grantee: Rebeka Tomasin
• Support type: Scholarships in Brazil - Doctorate