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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

An overview of the human brain myelin proteome and differences associated with schizophrenia

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Author(s):
Martins-de-Souza, Daniel [1, 2, 3, 4] ; Guest, Paul C. [4] ; Reis-de-Oliveira, Guilherme [4] ; Schmitt, Andrea [5] ; Falkai, Peter [5] ; Turck, Christoph W. [6]
Total Authors: 6
Affiliation:
[1] Conselho Nacl Desenvolvimento Cient & Tecnol, Inst Nacl Biomarcadores Neuropsiquiatria INBION, Sao Paulo - Brazil
[2] DOr Inst Res & Educ IDOR, Sao Paulo - Brazil
[3] Univ Estadual Campinas, Expt Med Res Cluster EMRC, Campinas - Brazil
[4] Univ Campinas UNICAMP, Lab Neuroprote, Dept Biochem & Tissue Biol, Inst Biol, Rua Monteiro Lobato 255, BR-13083862 Campinas, SP - Brazil
[5] Ludwig Maximilians Univ Munchen, Dept Psychiat & Psychotherapy, Munich - Germany
[6] Max Planck Inst Psychiat, Dept Translat Res Psychiat, Munich - Germany
Total Affiliations: 6
Document type: Journal article
Source: WORLD JOURNAL OF BIOLOGICAL PSYCHIATRY; v. 22, n. 4 JUL 2020.
Web of Science Citations: 0
Abstract

Objectives Disturbances in the myelin sheath drive disruptions in neural transmission and brain connectivity as seen in schizophrenia. Here, the myelin proteome was characterised in schizophrenia patients and healthy controls to visualise differences in proteomic profiles. Methods A liquid chromatography tandem mass spectrometry-based shotgun proteomic analysis was performed of a myelin-enriched fraction of postmortem brain samples from schizophrenia patients (n = 12) and mentally healthy controls (n = 8).In silicopathway analyses were performed on the resulting data. Results The present characterisation of the human myelinome led to the identification of 480 non-redundant proteins, of which 102 proteins are newly annotated to be associated with the myelinome. Levels of 172 of these proteins were altered between schizophrenia patients and controls. These proteins were mainly associated with glial cell differentiation, metabolism/energy, synaptic vesicle function and neurodegeneration. The hub proteins with the highest degree of connectivity in the network included multiple kinases and synaptic vesicle transport proteins. Conclusions Together these findings suggest disruptive effects on synaptic activity and therefore neural transmission and connectivity, consistent with the dysconnectivity hypothesis of schizophrenia. Further studies on these proteins may lead to the identification of potential drug targets related to the synaptic dysconnectivity in schizophrenia and other psychiatric and neurodegenerative disorders. (AU)

FAPESP's process: 19/00098-7 - Multi-User Equipment approved in grant 2017/25588-1: cromatógrafo Acquity UPLC I-Class
Grantee:Daniel Martins-de-Souza
Support Opportunities: Multi-user Equipment Program
FAPESP's process: 18/01410-1 - Evaluating the effects of clozapine on immune system cells
Grantee:Guilherme Reis-De-Oliveira
Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
FAPESP's process: 17/25588-1 - From the basic understanding to clinical biomarkers to schizophrenia: a neuroproteomics-centered multidisciplinary study
Grantee:Daniel Martins-de-Souza
Support Opportunities: Research Projects - Thematic Grants