| Full text | |
| Author(s): |
Maia Rocha Neto, Joao Batista
[1]
;
Soares, Andrey Coatrini
[2]
;
Bataglioli, Rogerio Aparecido
[1]
;
Carr, Olivia
[3]
;
Rodrigues Costa, Carlos Alberto
[4]
;
Oliveira Jr, Osvaldo N.
;
Beppu, Marisa Masumi
[5, 1]
;
Carvalho, Hernandes F.
[5, 6]
Total Authors: 8
|
| Affiliation: | [1] Univ Estadual Campinas, Sch Chem Engn, BR-13083852 Campinas - Brazil
[2] Embrapa Instrumentat, Natl Lab Nanotechnol Agribusiness LNNA, BR-13560970 Sao Carlos - Brazil
[3] Univ Sao Paulo, Sao Carlos Inst Phys, BR-13566590 Sao Carlos - Brazil
[4] Brazilian Ctr Res Energy & Mat CNPEM, Brazilian Nanotechnol Natl Lab LNNano, BR-13083970 Campinas, SP - Brazil
[5] Univ Estadual Campinas, Inst Biol, BR-13083970 Campinas - Brazil
[6] Natl Inst Photon Appl Cell Biol INFABIC, BR-13083859 Campinas - Brazil
Total Affiliations: 6
|
| Document type: | Journal article |
| Source: | CELLS; v. 9, n. 6 JUN 2020. |
| Web of Science Citations: | 0 |
| Abstract | |
The increasing need for point-of-care diagnosis has sparked the development of label-free sensing platforms, some of which are based on impedance measurements with biological cells. Here, interdigitated electrodes were functionalized with layer-by-layer (LbL) films of hyaluronan (HA) and chitosan (CHI) to detect prostatic tumor cells (PC3 line). The deposition of LbL films was confirmed with atomic force microscopy and polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS), which featured the vibrational modes of the HA top layer capable of interacting specifically with glycoprotein CD44 receptors overexpressed in tumor cells. Though the CHI/HA LbL films cannot be considered as a traditional biosensor due to their limited selectivity, it was possible to distinguish prostate tumor cells in the range from 50 to 600 cells/mu L in in vitro experiments with impedance spectroscopy. This was achieved by treating the impedance data with information visualization methods, which confirmed the distinguishing ability of the films by observing the absence of false positives in a series of control experiments. The CD44-HA interactions may, therefore, be exploited in clinical analyses and point-of-care diagnostics for cancer, particularly if computational methods are used to process the data. (AU) | |
| FAPESP's process: | 18/22214-6 - Towards a convergence of technologies: from sensing and biosensing to information visualization and machine learning for data analysis in clinical diagnosis |
| Grantee: | Osvaldo Novais de Oliveira Junior |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 18/20560-4 - Development of bioactive materials based on proteins and natural polymers aiming applications of biomedical interest. |
| Grantee: | Marisa Masumi Beppu |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 18/18953-8 - Nanostructured films applied in microfluidic biosensors to mastitis detection |
| Grantee: | Andrey Coatrini Soares |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 16/10193-9 - New mechanisms for controlling triggered release of anticancer drugs in cellular backpacks |
| Grantee: | Rogério Aparecido Bataglioli |
| Support Opportunities: | Scholarships in Brazil - Doctorate (Direct) |
| FAPESP's process: | 14/50938-8 - INCT 2014: in Photonics Applied to Cell Biology |
| Grantee: | Hernandes Faustino de Carvalho |
| Support Opportunities: | Research Projects - Thematic Grants |