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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Aminofunctionalized LAPONITE (R) as a versatile hybrid material for chlorhexidine digluconate incorporation: Cytotoxicity and antimicrobial activities

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Author(s):
Peraro, Gustavo R. [1] ; Donzelli, Eduardo H. [1] ; Oliveira, Pollyanna F. [1] ; Tavares, Denise Crispim [1] ; Martins, Carlos H. Gomes [1] ; Molina, Eduardo F. [1] ; de Faria, Emerson H. [1]
Total Authors: 7
Affiliation:
[1] Univ Franca, UNIFRAN, Grp Pesquisa Mat Lamelares Hibridos, Ave Dr Armando Salles Oliveira, 201, Parque Univ, BR-14404600 Franca, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: APPLIED CLAY SCIENCE; v. 195, SEP 15 2020.
Web of Science Citations: 0
Abstract

Aminofunctionalized LAPONITE (R); RD was employed for efficient chlorhexidine digluconate (CHX-DG) in-corporation. The structure and properties of the unloaded (Lap-APTES) and loaded (Lap-APTES:CHX-DG) hy-brids were investigated by powder X-ray diffraction (PXRD), thermal analysis, small angle X-ray scattering (SAXS), and infrared absorption spectroscopy (FTIR). A typical adsorption experiment was conducted to evaluate how the parameters contact time and concentration affected CHX-DG incorporation into the clay and showed that this compound had high affinity for Lap-APTES, adsorption equilibrium was reached at 90 min, with CHX-DG incorporation of 1402.7 mg/g. FTIR analysis confirm the interaction by hydrogen bonds between the CHX-DG and APTES amine groups and the laponite OH groups, resulting in materials with potential antibacterial properties for controlled drug release. Even at low concentrations (0.042 mg/g), Lap-APTES:CHX-DG was effective against S. pyogenes. The Lap, Lap-APTES, and Lap-APTES:CHX-DG cytotoxicity to GM07492A cells (human fibroblasts) was investigated by the XTT colorimetric assay, which revealed that CHX-DG incorporation into Lap-APTES reduced drug cytotoxicity. Drug release experiments demonstrated that a maximum of 10% (m/m) CHX-DG was released within 24 h and confirmed the higher affinity between Lap-APTES and CHX-DG. (AU)

FAPESP's process: 13/19523-3 - Functional Biohybrid materials based on layered and/or fibrous natural clays with multifaceted applications
Grantee:Emerson Henrique de Faria
Support Opportunities: Regular Research Grants
FAPESP's process: 13/20455-2 - Nanostructured organic-inorganic hybrids for controlled lignans release with pharmacological potential
Grantee:Eduardo Ferreira Molina
Support Opportunities: Research Grants - Young Investigators Grants
FAPESP's process: 17/15482-1 - Novel strategies synthesis to reuse Tanner industry by-products to obtain high-value functional porous materials
Grantee:Emerson Henrique de Faria
Support Opportunities: Regular Research Grants