Progesterone decreases ovarian cancer cells migration and invasion

Author(s)

Lima, Maira A. ^[1] ; Silva, V, Suely ; Jaeger, Ruy G. ^[2] ; Freitas, Vanessa M. ^[2]

Affiliation

^[1] Univ Sao Paulo, Inst Ciencias Biomed, Dept Biol Celular & Desenvolvimento, Av Prof Lineu Prestes 1524, Ed Biomed 1 Sala 428, BR-05508000 Sao Paulo, SP - Brazil

^[2] Silva, Suely, V, Univ Sao Paulo, Inst Ciencias Biomed, Dept Biol Celular & Desenvolvimento, Av Prof Lineu Prestes 1524, Ed Biomed 1 Sala 428, BR-05508000 Sao Paulo, SP - Brazil

• Document type: Journal article
• Source: Steroids; v. 161, SEP 2020.
• Web of Science Citations: 0

Abstract

The progression of cancer depends on the interaction between the cells and their microenvironment. Progesterone is a steroid and progestogen sex hormone produced by the corpus luteum, which is a transitory endocrine gland in female mammals and prepares the endometrium for implantation. Also, progesterone is involved in antitumorigenic process in different types of cancer. Our goal is to investigate the role of progesterone in cell invasion and migration. Ovarian cells were treated with different concentrations of progesterone. 500 nM or 1 mu M progesterone decreased the migration of the cells in 24 h or less without affecting the viability. Immunoblot showed that treatment with 1 mu M progesterone decreased the phosphorylated forms of Src and FAK, and the cells were less polarized. Our results suggest that progesterone interferes with migration and invasion of ovarian cells. Inhibitory experiments inferred the progesterone receptor playing a role in migration and invasion. Decreased phosphorylation of molecules involved in these processes was also found. (AU)

• FAPESP's process: 18/19813-5 - Function of progesterone and ADAMTS 1 in the migration of cells derived from ovarian cancer
• Grantee: Maíra de Assis Lima
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• FAPESP's process: 18/05566-6 - Nuclear ADAMTS-1 regulating normal and cancer cells
• Grantee: Vanessa Morais Freitas
• Support Opportunities: Regular Research Grants
• FAPESP's process: 15/19773-5 - Functions of progesterone and ADAMTS 1 protease in the migration of cells derived from ovarian cancer
• Grantee: Maíra de Assis Lima
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 10/07699-1 - Protease ADAMTS1 influencing breast cancer behavior and microenvironment
• Grantee: Vanessa Morais Freitas
• Support Opportunities: Research Grants - Young Investigators Grants