Utrophin, MHC and M1/M2 macrophages in GRMD dogs

Author(s)

Gabriela Noronha de Toledo ^[1] ; Julieta Rodini Engracia de Moraes ^[2]

Affiliation

^[1] Universidade Federal de Campina Grande. Centro de Saúde e Tecnologia Rural - Brasil

^[2] Universidade Estadual de São Paulo "Julio de Mesquita Filho". Faculdade de Ciências Agrárias e Veterinárias. Departamento de Patologia Veterinária - Brasil

• Document type: Journal article
• Source: Ciênc. anim. bras.; v. 21, 2020-08-19.

Abstract

Abstract Muscular dystrophies are hereditary diseases that lead to progressive degeneration of the skeletal musculature. Golden Retriever dogs are used as animal models because they show a hereditary muscle disease similar to muscular dystrophy in humans. Aims: To evaluate the immunostaining of M1 (CD68) and M2 (CD163) macrophages, MHC I, MHC II and, utrophin in muscles of Golden Retriever dogs affected by muscular dystrophy (GRMD). Methods: Samples from 17 male dogs affected by GRMD were divided into GI - dystrophic dogs up to one year of age; and GII - dystrophic dogs over one-year-old. Results: Immunostaining of CD163 was higher than CD68 in both GI and GII. CD68 showed no variation between groups of dystrophic animals. MHC class I immunostaining was most evident in the biceps femoris and triceps brachialis. MHC class II was expressed mildly in four dystrophic muscle types in GI and GII. Utrophin immunostaining was higher in GII. Conclusion: M2 macrophages were one of the main mononuclear inflammatory cells found in dystrophic muscles. The number of M2 in muscles of dogs with GRMD increases with age, linking this cell subtype to permanent muscle damage. (AU)

• FAPESP's process: 11/07906-0 - Expression of utrophin, CD68 and CD163 in skeletal muscle of dogs affected by progressive muscular dystrophy
• Grantee: Julieta Rodini Engracia de Moraes
• Support Opportunities: Regular Research Grants