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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Evidence of macrophage modulation in the mouse pubic symphysis remodeling during the end of first pregnancy and postpartum

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Castelucci, B. G. [1] ; Pereira, A. H. M. [2] ; Fioramonte, M. [3] ; Carazzolle, M. F. [4] ; de Oliveira, P. S. L. [2] ; Franchini, K. G. [2] ; Kobarg, J. [5] ; Martins-de-Souza, D. [6, 3, 7] ; Joazeiro, P. P. [1] ; Consonni, S. R. [1]
Total Authors: 10
[1] State Univ Campinas UNICAMP, Inst Biol, Dept Biochem & Tissue Biol, Lab Cytochem & Immunocytochem, Campinas - Brazil
[2] Brazilian Ctr Res Energy & Mat CNPEM, Brazilian Biosci Natl Lab, Campinas - Brazil
[3] State Univ Campinas UNICAMP, Inst Biol, Dept Biochem & Tissue Biol, Lab Neuroprote, Campinas - Brazil
[4] State Univ Campinas UNICAMP, Dept Genet Evolut Microbiol & Immunol, Inst Biol, Campinas - Brazil
[5] State Univ Campinas UNICAMP, Sch Pharmaceut Sci, Campinas - Brazil
[6] DOr Inst Res & Educ IDOR, Sao Paulo - Brazil
[7] State Univ Campinas UNICAMP, Expt Med Res Cluster EMRC, Campinas - Brazil
Total Affiliations: 7
Document type: Journal article
Source: SCIENTIFIC REPORTS; v. 10, n. 1 JUL 24 2020.
Web of Science Citations: 0

In mouse pregnancy, pubic symphysis (PS) remodels into an elastic interpubic ligament (IpL) in a temporally regulated process to provide safe delivery. It restores at postpartum to assure reproductive tract homeostasis. Recently, macrophage localization in the IpL and dynamic changes in the expression of inflammatory mediators observed from the end of pregnancy (D18, D19) to early days postpartum (1dpp, 3dpp) highlighted the necessity of the identification of the key molecules involved in innate immune processes in PS remodeling. Therefore, this study uses morphological and high-sensitivity molecular techniques to identify both macrophage association with extracellular matrix (ECM) remodeling and the immunological processes involved in PS changes from D18 to 3dpp. Results showed macrophage association with active gelatinases and ECM components and 25 differentially expressed genes (DEGs) related to macrophage activities in interpubic tissues from D18 to 3dpp. Additionally, microarray and proteomic analysis showed a significant association of interpubic tissue DEGs with complement system activation and differentially expressed proteins (DEPs) with phagocytosis, highlighting the involvement of macrophage-related activities in mouse PS remodeling. Therefore, the findings suggest that PS ECM remodeling is associated with evidence of macrophage modulation that ensures both IpL relaxation and fast PS recovery postpartum for first labor. (AU)

FAPESP's process: 15/23616-2 - Characterization of monocytes and macrophages activation states present in the mouse pubic symphysis remodeling at late pregnancy and postpartum.
Grantee:Paulo Pinto Joazeiro
Support type: Regular Research Grants
FAPESP's process: 17/03489-1 - From functional studies to searching for new inhibitors for cancer: exploring kinases that regulate the cell cycle of the human NEK family
Grantee:Jörg Kobarg
Support type: Research Projects - Thematic Grants