| Full text | |
| Author(s): |
Kenzo-Kagawa, Bruno
[1]
;
Vieira, Willians Fernando
[2, 1]
;
Cogo, Jose Carlos
[3]
;
da Cruz-Hofling, Maria Alice
[1]
Total Authors: 4
|
| Affiliation: | [1] State Univ Campinas UNICAMP, Inst Biol, Dept Biochem & Tissue Biol, Rua Monteiro Lobato 255, BR-13083970 Campinas, SP - Brazil
[2] State Univ Campinas UNICAMP, Inst Biol, Dept Struct & Funct Biol, Campinas, SP - Brazil
[3] Brazil Univ, Fac Biomed Engn, Itaquera - Brazil
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | Toxicology and Applied Pharmacology; v. 402, SEP 1 2020. |
| Web of Science Citations: | 0 |
| Abstract | |
Bites by viperid snakes belonging to Bothrops genus produce fast and intense local edema, inflammation, bleeding and myonecrosis. In this study, we investigated the role of Myogenic Regulatory Factors (MRFs: MyoD; Myog), negatively regulated by GDF-8 (Myostatin), and ubiquitin-proteasome system pathway (UPS: MuRF-1; Fbx-32) in gastrocnemius muscle regeneration after Bothrops jararacussu snake venom (Bjussu) or its isolated phospholipase A(2) myotoxins, BthTx-I (Lys-49 PLA(2)) and BthTx-II (Asp-49 PLA(2)) injection. Male Swiss mice received a single infra-gastrocnemius injection of crude Bjussu, at a dose/volume of 0.83 mg/kg/20 mu l, and BthTx-I or BthTx-II, at a dose/volume of 2.5 mg/kg/20 mu l. Control mice (Sham) received an injection of sterile saline solution (NaCl 0.9%; 20 mu l). At 24, 48, 72 and 96 h post injection, right gastrocnemius was collected for protein expression analyses. Based on the temporal expressional dynamics of MyoD, Myog and GDF-8/Myostatin, it was possible to propose that the myogenesis pathway was impacted most badly by BthTx-II followed by BthTx-I and lastly by B. jararacussu venom, thus suggesting that catalytic activity has likely inhibitory role on the satellite cells-mediated reparative myogenesis pathway. Inversely, the catalytic activity seems to be not a determinant for the activation of proteins ubiquitination by MuRF-1 and Fbx-32/Atrogin-1 E3 proteasome ligases, given proteolysis pathway through UPS was activated neither after Bjussu, nor after BthTx-II, but just after the catalytically-inactive BthTx-I Lys-49 PLA(2)-homologue exposure. The findings of this study disclose interesting perspective for further mechanistic studies about pathways that take part in the atrophy and repair after permanent damage induced by bothropic snakebites. (AU) | |