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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Copaiferaoleoresins as a novel natural product against acanthocephalan in aquaculture: Insights in the mode of action and toxicity

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Author(s):
de Seixas, Arthur Tavares [1] ; Gallani, Silvia Umeda [1] ; Noronha, Leilane da Silva [2] ; Mangabeira Silva, Jonas Joaquim [3] ; Rizzato Paschoal, Jonas Augusto [3] ; Bastos, Jairo Kenupp [3] ; Ramos Valladao, Gustavo Moraes [1]
Total Authors: 7
Affiliation:
[1] Nilton Lins Univ, Postgrad Program Aquaculture, Manaus, Amazonas - Brazil
[2] Nilton Lins Univ, Pharm, Manaus, Amazonas - Brazil
[3] Univ Sao Paulo, Sch Pharmaceut Sci, Ribeirao Preto - Brazil
Total Affiliations: 3
Document type: Journal article
Source: AQUACULTURE RESEARCH; v. 51, n. 11 AUG 2020.
Web of Science Citations: 0
Abstract

TambaquiColossoma macropomumis the main South American fish in continental aquaculture. However, its production has been hampered by acanthocephalanNeoechinorhynchus buttnerae. In this study, we evaluated the antiparasitic potential of five oleoresins (ORs):Copaifera duckei,Copaifera pubiflora,Copaifera reticulata,Copaifera pauperaandCopaifera multijuga, as a novel treatment againstN. buttnerae, describing their mode of action by histology of the parasite and their toxicity to the host. In in vitro assays, parasites were exposed to three concentrations (0.0467, 0.0934 and 0.1868 mg/ml) of ORs. At the lowest concentration tested (0.0467 mg/ml), none of the ORs had a significant effect on acanthocephalans (p > .05). At the intermediate concentration (0.0934 mg/ml),C. duckei,C. pubifloraandC. reticulatawere significantly (p < .05) more effective thanC. pauperaandC. multijuga. At the highest concentration (0.1868 mg/ml), all species ofCopaiferaresulted in 100% mortality of the parasites after 24 hr, exceptC. multijuga. Acanthocephalans killed by the ORs showed generalized vacuolization in the tissues with abnormal accumulation of fluid (remarkable body swelling). We were able to relate the antiparasitic effect of ORs to their composition (ORs with highest concentration of diterpenes were most effective). In toxicity testing, tambaqui were exposed to the three most effective ORs (C. duckei,C. pubifloraandC. reticulata). All were classified as moderately toxic. In vitro and in vivo tests showed that Amazon ORs have the potential to be included in a list of products for therapeutic purposes in aquaculture. (AU)

FAPESP's process: 11/13630-7 - Chemical and pharmacological validation of extracts and active compounds of Copaifera species
Grantee:Jairo Kenupp Bastos
Support Opportunities: Research Projects - Thematic Grants