Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

A transcriptome and proteome of the tick Rhipicephalus microplus shaped by the genetic composition of its hosts and developmental stage

Full text
Author(s):
Garcia, Gustavo R. [1, 2] ; Ribeiro, Jose Marcos Chaves [3] ; Maruyama, Sandra Regina [4, 1] ; Gardinassi, Luiz Gustavo [5, 1] ; Nelson, Kristina [6] ; Ferreira, Beatriz R. [1, 7] ; Andrade, Thales Galdino [1, 7] ; Ferreira de Miranda Santos, Isabel K. [1]
Total Authors: 8
Affiliation:
[1] Univ Sao Paulo, Ribeirao Preto Sch Med, Dept Biochem & Immunol, Ave Bandeirantes 3900, BR-14049900 Ribeirao Preto, SP - Brazil
[2] Superintendence Sao Paulo State Tech & Sci Police, Sao Paulo, SP - Brazil
[3] NIAID, Lab Malaria & Vector Res, NIH, 9000 Rockville Pike, Bethesda, MD 20892 - USA
[4] Univ Fed Sao Carlos, Ctr Biol Sci & Hlth, Dept Genet & Evolut, Sao Carlos, SP - Brazil
[5] Univ Fed Goias, Inst Trop Pathol & Publ Hlth, Goiania, Go - Brazil
[6] Virginia Commonwealth Univ, Ctr Study Biol Complex, Richmond, VA - USA
[7] Univ Sao Paulo, Ribeirao Preto Sch Nursing, Dept Maternal Child Nursing & Publ Hlth, Ribeirao Preto, SP - Brazil
Total Affiliations: 7
Document type: Journal article
Source: SCIENTIFIC REPORTS; v. 10, n. 1 JUL 30 2020.
Web of Science Citations: 4
Abstract

The cattle tick, Rhipicephalus microplus, is a monoxenous tick that co-evolved with indicine cattle on the Indian subcontinent. It causes massive damage to livestock worldwide. Cattle breeds present heritable, contrasting phenotypes of tick loads, taurine breeds carrying higher loads of the parasite than indicine breeds. Thus, a useful model is available to analyze mechanisms that determine outcomes of parasitism. We sought to gain insights on these mechanisms and used RNA sequencing and Multidimensional Protein Identification Technology (MudPIT) to generate a transcriptome from whole larvae and salivary glands from nymphs, males and females feeding on genetically susceptible and resistant bovine hosts and their corresponding proteomes. 931,698 reads were annotated into 11,676 coding sequences (CDS), which were manually curated into 116 different protein families. Male ticks presented the most diverse armamentarium of mediators of parasitism. In addition, levels of expression of many genes encoding mediators of parasitism were significantly associated with the level and stage of host immunity and/or were temporally restricted to developmental stages of the tick. These insights should assist in developing novel, sustainable technologies for tick control. (AU)

FAPESP's process: 13/00382-0 - PROSPECTING TICK SIALOMES FOR DISCOVERY OF NEW ANTIMICROBIAL COMPONENTS
Grantee:Gustavo Rocha Garcia
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 12/04087-0 - RNA-seq of bovine leukocytes from peripheral blood: a functional analysis of the transcriptome in cattle immunized with recombinant antigens from the cattle tick, Rhipicephalus microplus, and in infested cattle
Grantee:Sandra Regina Costa Maruyama
Support Opportunities: Scholarships in Brazil - Post-Doctoral