Differential gene expression elicited by ZIKV infection in trophoblasts from congenital Zika syndrome discordant twins

Author(s):Show less -

Amaral, Murilo Sena ^[1] ; Goulart, Ernesto ^[2] ; Caires-Junior, Luiz Carlos ^[2] ; Morales-Vicente, David Abraham ^{[3, 1]} ; Soares-Schanoski, Alessandra ^[4] ; Gomes, Roselane Paiva ^[5] ; Olberg, Giovanna Goncalves de Oliveira ^[1] ; Astray, Renato Mancini ^[5] ; Kalil, Jorge E. ^{[6, 7]} ; Zatz, Mayana ^[2] ; Verjovski-Almeida, Sergio ^{[3, 1]}

Affiliation

^[1] Inst Butantan, Lab Parasitol, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Inst Biociencias, Ctr Pesquisas Genoma Humano & Celulas Tronco, Dept Genet & Biol Evolut, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Inst Quim, Dept Bioquim, Sao Paulo - Brazil

^[4] Inst Butantan, Lab Bacteriol, Sao Paulo - Brazil

^[5] Inst Butantan, Lab Imunol Viral, Sao Paulo - Brazil

^[6] Univ Sao Paulo, Inst Coracao, Sao Paulo - Brazil

^[7] Univ Sao Paulo, Fac Med, Lab Imunol, Sao Paulo - Brazil

• Document type: Journal article
• Source: PLoS Neglected Tropical Diseases; v. 14, n. 8 AUG 2020.
• Web of Science Citations: 0

Abstract

Zika virus (ZIKV) causes congenital Zika syndrome (CZS), which is characterized by fetal demise, microcephaly and other abnormalities. ZIKV in the pregnant woman circulation must cross the placental barrier that includes fetal endothelial cells and trophoblasts, in order to reach the fetus. CZS occurs in similar to 1-40% of cases of pregnant women infected by ZIKV, suggesting that mothers' infection by ZIKV during pregnancy is not deterministic for CZS phenotype in the fetus. Therefore, other susceptibility factors might be involved, including the host genetic background. We have previously shown that in three pairs of dizygotic twins discordant for CZS, neural progenitor cells (NPCs) from the CZS-affected twins presented differentialin vitroZIKV susceptibility compared with NPCs from the non-affected. Here, we analyzed human-induced-pluripotent-stem-cell-derived (hiPSC-derived) trophoblasts from these twins and compared by RNA-Seq the trophoblasts from CZS-affected and non-affected twins. Followingin vitroexposure to a Brazilian ZIKV strain (ZIKV(BR)), trophoblasts from CZS-affected twins were significantly more susceptible to ZIKV(BR) infection when compared with trophoblasts from the non-affected. Transcriptome profiling revealed no differences in gene expression levels of ZIKV candidate attachment factors, IFN receptors and IFN in the trophoblasts, either before or after ZIKV(BR) infection. Most importantly, ZIKV(BR) infection caused, only in the trophoblasts from CZS-affected twins, the downregulation of genes related to extracellular matrix organization and to leukocyte activation, which are important for trophoblast adhesion and immune response activation. In addition, only trophoblasts from non-affected twins secreted significantly increased amounts of chemokines RANTES/CCL5 and IP10 after infection with ZIKV(BR). Overall, our results showed that trophoblasts from non-affected twins have the ability to more efficiently activate genes that are known to play important roles in cell adhesion and in triggering the immune response to ZIKV infection in the placenta, and this may contribute to predict protection from ZIKV dissemination into fetuses' tissues. Author summary The Zika virus (ZIKV) infection in adults is usually characterized by mild flu-like symptoms, with most cases remaining asymptomatic. However, in the last years, widespread ZIKV infection was shown for the first time to be associated with congenital Zika syndrome (CZS) and death of neonates. It is estimated that CZS occurs in similar to 1-40% of cases of pregnant women infected by ZIKV, which suggests that different susceptibility factors might be involved, including the host genetic background. Here, by analyzing trophoblast cells that recapitulate the placenta from three pairs of dizygotic twins discordant for CZS, we were able to show that trophoblasts from CZS-affected twins were significantly more susceptible to ZIKV infection when compared with trophoblasts from the non-affected twins. We also provide a detailed picture of genes differentially expressed by trophoblasts from the discordant twins after infection with ZIKV. These genes can be further investigated as possible therapeutic targets to avoid viral dissemination into developing fetus' tissues. Our results suggest that CZS might be caused, among other factors, by a decreased ability of the placenta to respond to ZIKV infection in CZS-affected neonates, concomitant with a previously known deregulation of neural development genes in ZIKV-infected neuroprogenitor cells of these CZS-affected babies. (AU)

• FAPESP's process: 14/03620-2 - Characterization of the mechanisms of action of long non-coding RNAs involved with gene activation programs in human cells
• Grantee: Sergio Verjovski Almeida
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 13/08028-1 - CEGH-CEL - Human Genome and Stem Cell Research Center
• Grantee: Mayana Zatz
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC
• FAPESP's process: 14/50931-3 - Aging and genetic disorders: genomics and metagenomics
• Grantee: Mayana Zatz
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 18/23693-5 - Mechanisms of action of long non-coding RNAs involved with gene activation programs in eukaryotes
• Grantee: Sergio Verjovski Almeida
• Support Opportunities: Research Projects - Thematic Grants