| Full text | |
| Author(s): |
Pereira, Luiz Miguel
[1, 2]
;
Mota, Caroline Martins
[3]
;
Baroni, Luciana
[2]
;
Bronzon da Costa, Cassia Mariana
[2]
;
Venancio Brochi, Jade Cabestre
[2]
;
Wainwright, Mark
[4]
;
Patriarca Mineo, Tiago Wilson
[3]
;
Leite Braga, Gilberto Ubida
[2]
;
Yatsuda, Ana Patricia
[1, 2]
Total Authors: 9
|
| Affiliation: | [1] Univ Sao Paulo, Nucleo Apoio Pesquisa Prod Nat & Sintet, Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Av Cafe Sn-N, BR-14040903 Ribeirao Preto, SP - Brazil
[3] Univ Fed Uberlandia, Inst Biomed Sci, Dept Immunol, Uberlandia, MG - Brazil
[4] Liverpool John Moores Univ, Sch Pharm & Biomol Sci, Liverpool L3 3AF, Merseyside - England
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | SCIENTIFIC REPORTS; v. 10, n. 1 MAY 4 2020. |
| Web of Science Citations: | 0 |
| Abstract | |
Neospora caninum is an Apicomplexan parasite related to important losses in livestock, causing abortions and decreased fertility in affected cows. Several chemotherapeutic strategies have been developed for disease control; however, no commercial treatment is available. Among the candidate drugs against neosporosis, phenothiazinium dyes, offer a low cost-efficient approach to parasite control. We report the anti-parasitic effects of the phenothiaziums Methylene Blue (MB), New Methylene Blue (NMB), 1,9-Dimethyl Methylene Blue (DMMB) and Toluidine Blue O (TBO) on N. caninum, using in vitro and in vivo models. The dyes inhibited parasite proliferation at nanomolar concentrations (0.019-1.83 mu M) and a synergistic effect was achieved when Methylene Blue was combined with New Methylene Blue (Combination Index = 0.84). Moreover, the phenothiazinium dyes improved parasite clearance when combined with Pyrimethamine (Pyr). Combination of Methylene Blue + 1,9-Dimethyl Methylene Blue demonstrated superior efficacy compared to Pyrimethamine based counterparts in an in vivo model of infection. We also observed that Methylene Blue, New Methylene Blue and 1,9-Dimethyl Methylene Blue increased by 5000% the reactive oxygen species (ROS) levels in N. caninum tachyzoites. Phenothiazinium dyes represent an accessible group of candidates with the potential to compound future formulations for neosporosis control. (AU) | |
| FAPESP's process: | 16/11386-5 - Mechanistic study of perception and response to visible light in the entomopathogenic fungus Metarhizium acridum |
| Grantee: | Gilberto Úbida Leite Braga |
| Support Opportunities: | Regular Research Grants |