Mass spectrometry imaging as a potential technique for diagnostic of Huanglongbing disease using fast and simple sample preparation

Author(s)

de Moraes Pontes, Joao Guilherme ^[1] ; Vendramini, Pedro Henrique ^{[2, 3]} ; Fernandes, Laura Soler ^[1] ; de Souza, Fabricio Henrique ^[4] ; Pilau, Eduardo Jorge ^[5] ; Eberlin, Marcos Nogueira ^{[2, 3]} ; Magnani, Rodrigo Facchini ^[6] ; Wulff, Nelson Arno ^[6] ; Fill, Taicia Pacheco ^[1]

Affiliation

^[1] IQ UNICAMP, Lab Biol Quim Microbiana LaBioQuiMi, Campinas, SP - Brazil

^[2] IQ UNICAMP, ThoMSon Mass Spectrometry Lab, Campinas, SP - Brazil

^[3] Univ Prebiteriana Mackenzie, Sch Engn PPGEMN, MackMass Lab, Sao Paulo, SP - Brazil

^[4] Univ Estadual Maringa, Programa Posgrad Ciencias Biol, Maringa, Parana - Brazil

^[5] Univ Estadual Maringa, Dept Quim, Lab Biomol & Espectrometria Massas LabioMass, Maringa, Parana - Brazil

^[6] Fundo Def Citricultura FUNDECITRUS, Dept Pesquisa & Desenvolvimento, Araraquara, SP - Brazil

• Document type: Journal article
• Source: SCIENTIFIC REPORTS; v. 10, n. 1 AUG 10 2020.
• Web of Science Citations: 5

Abstract

Huanglongbing (HLB) is a disease of worldwide incidence that affects orange trees, among other commercial varieties, implicating in great losses to the citrus industry. The disease is transmitted through Diaphorina citri vector, which inoculates Candidatus Liberibacter spp. in the plant sap. HLB disease lead to blotchy mottle and fruit deformation, among other characteristic symptoms, which induce fruit drop and affect negatively the juice quality. Nowadays, the disease is controlled by eradication of sick, symptomatic plants, coupled with psyllid control. Polymerase chain reaction (PCR) is the technique most used to diagnose the disease; however, this methodology involves high cost and extensive sample preparation. Mass spectrometry imaging (MSI) technique is a fast and easily handled sample analysis that, in the case of Huanglongbing allows the detection of increased concentration of metabolites associated to the disease, including quinic acid, phenylalanine, nobiletin and sucrose. The metabolites abieta-8,11,13-trien-18-oic acid, suggested by global natural product social molecular networking (GNPS) analysis, and 4-acetyl-1-methylcyclohexene showed a higher distribution in symptomatic leaves and have been directly associated to HLB disease. Desorption electrospray ionization coupled to mass spectrometry imaging (DESI-MSI) allows the rapid and efficient detection of biomarkers in sweet oranges infected with Candidatus Liberibacter asiaticus and can be developed into a real-time, fast-diagnostic technique. (AU)

• FAPESP's process: 19/06359-7 - Understanding the chemical factors involved in the endophytism x pathogenicity of different Penicillium brasilianum isolates
• Grantee: Taicia Pacheco Fill
• Support Opportunities: Regular Research Grants
• FAPESP's process: 17/24462-4 - Understanding the virulence strategies of Penicillium digitatum against the host citrus
• Grantee: Taicia Pacheco Fill
• Support Opportunities: Regular Research Grants
• FAPESP's process: 18/03670-0 - Prospecting for anticancer secondary metabolites produced by phytopathogenic fungi
• Grantee: Jaqueline Moraes Bazioli
• Support Opportunities: Scholarships in Brazil - Master