| Full text | |
| Author(s): |
Yokomichi, Anna Laura Yuri
[1]
;
Rodrigues, Valquiria da Cruz
[2]
;
Moroz, Andrei
[3]
;
Bertanha, Matheus
[4]
;
Ribeiro, Sidney J. L.
[3]
;
Deffune, Elenice
[4]
;
Moraes, Marli L.
[1]
Total Authors: 7
|
| Affiliation: | [1] Univ Fed Sao Paulo, Inst Ciencia & Tecnol, Sao Jose Dos Campos, SP - Brazil
[2] Univ Sao Paulo, Inst Fis Sao Carlos, Sao Carlos, SP - Brazil
[3] Univ Estadual Paulista, Inst Quim, Araraquara, SP - Brazil
[4] Univ Estadual Paulista, Hemoctr Botucatu, Botucatu, SP - Brazil
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | Talanta; v. 219, NOV 1 2020. |
| Web of Science Citations: | 0 |
| Abstract | |
Venous thromboembolism (VTE) is a serious clinical condition which early and accurate diagnosis may contribute to the reduction of associated morbidity and mortality. VTE occurs when a blood clot (thrombus) blocks the vein blood flow causing deep vein thrombosis (DVT) and, when it migrates to the lungs, it may clog the pulmonary arteries characterizing pulmonary embolism (PE). Analysis using fibrin degradation products or D-dimer and coagulation factor VIII may assist early diagnosis of VTE. Thus, two immunosensors were built using layer-by-layer (LbL) films technique, one containing the anti-D-dimer immobilized on polyethylene imine (PEI) and another the anti-FVIII on silk fibroin (SF). Immunosensor response, the antigen-antibody specific interaction, was investigated using cyclic voltammetry. When immunosensors, PEI/anti-D-dimer and SF/anti-FVIII, were exposed to antigens, D-dimer and Factor VIII, the voltammograms area and current were significantly increased with increasing specific antigen concentration. The specific interaction was confirmed with control experiments, electrodes containing only PEI or SF, that no significant changes in the voltammogram responses were observed and principal component analysis confirmed these results. The films formation and response were verified using scanning electronic microscopy (SEM). The developed immunosensor seems to be a promising and effective early complementary exam to assist in the VTE diagnosis, through the combined response of two biomarkers very sensible. (AU) | |
| FAPESP's process: | 14/03145-2 - Immunosensor for the diagnosis of venous thromboembolism based on immobilization of the antibody on silk fibroin |
| Grantee: | Anna Laura Yuri Yokomichi |
| Support Opportunities: | Scholarships in Brazil - Scientific Initiation |