| Full text | |
| Author(s): |
Britto-Junior, Jose
[1]
;
Antunes, Natalicia J.
[1]
;
Campos, Rafael
[2, 3]
;
Sucupira, Mauro
[2]
;
Mendes, Gustavo Duarte
[4, 5]
;
Fernandes, Fernanda
[4]
;
Moraes, Manoel Odorico
[2]
;
Moraes, Maria Elisabete A.
[2]
;
De Nucci, Gilberto
[6, 4, 2, 1]
Total Authors: 9
|
| Affiliation: | [1] State Univ Campinas UNICAMP, Dept Pharmacol, Fac Med Sci, Alexander Fleming St 105, BR-13083881 Campinas, SP - Brazil
[2] Fed Univ Ceara UFC, Drug Res & Dev Ctr, Clin Pharmacol Unit, Fortaleza, CE - Brazil
[3] Ceara State Univ UECE, Super Inst Biomed Sci, Fortaleza, CE - Brazil
[4] Sao Leopoldo Mand, Dept Pharmacol, Fac Med, Campinas, SP - Brazil
[5] Univ Metropolitana Santos, Dept Pharmacol, Fac Med, Santos, SP - Brazil
[6] Univ Sao Paulo, Inst Biomed Sci, Sao Paulo - Brazil
Total Affiliations: 6
|
| Document type: | Journal article |
| Source: | BIOMEDICAL CHROMATOGRAPHY; v. 35, n. 2 SEP 2020. |
| Web of Science Citations: | 1 |
| Abstract | |
This study presented for the first time the development and validation of a sensitive method for quantification of dopamine, noradrenaline, and adrenaline in Krebs-Henseleit solution by LC-tandem mass spectrometry. Aliquots of 2.0 mL calibrators, quality controls, and samples of Krebs-Henseleit solution incubated with tortoise's aortic ring for 30 min were extracted by solid-phase extraction. Catecholamine separation was achieved on a 100 x 4.6 mm LiChrospher RP-8 column and the quantification was performed by a mass spectrometer equipped with an electrospray interface operating in positive ion mode. The run time was 4 min and the calibration curve was linear over the range of 0.1-20.0 ng/mL. The method was applied to the measurement of basal release of dopamine, noradrenaline, and adrenaline from the tortoiseChelonoidis carbonariaaortaein vitro.One aortic ring (30 mm) per tortoise (n = 5) was incubated for 30 min in a 5 mL organ bath filled with Krebs-Henseleit solution. The method demonstrated sensitivity, precision, and accuracy enough for its application in the measurement of basal release of these catecholamines fromC. carbonariaaortic ringsin vitro.The mean (standard deviation) concentrations of dopamine, noradrenaline, and adrenaline were 3.48 (2.55) ng/mL, 1.40 (0.57) ng/mL, and 1.87 (1.09) ng/mL, respectively. (AU) | |
| FAPESP's process: | 16/22506-1 - Metabolism of dapaconazole |
| Grantee: | Natalícia de Jesus Antunes |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |