Optical clearing agent increases effectiveness of photodynamic therapy in a mouse model of cutaneous melanoma: an analysis by Raman microspectroscopy

Author(s)

Martinelli, Leticia Palombo ^{[1, 2]} ; Iermak, Ievgeniia ^[1] ; Moriyama, Lilian Tan ^[1] ; Requena, Michelle Barreto ^[1] ; Pires, Layla ^[3] ; Kurachi, Cristina ^{[1, 2]}

Affiliation

^[1] Univ Sao Paulo, Sao Carlos Inst Phys, Ave Trabalhador Sao Carlense 400, BR-13566590 Sao Carlos, SP - Brazil

^[2] Univ Fed Sao Carlos, Postgrad Program Biotechnol, Rodovia Washington Luis Km 235, BR-13565905 Sao Carlos - Brazil

^[3] Univ Hlth Network, Princess Margaret Canc Ctr, Princess Margaret Canc Res Tower, 101 Coll St, Toronto, ON M5G 1L7 - Canada

• Document type: Journal article
• Source: BIOMEDICAL OPTICS EXPRESS; v. 11, n. 11, p. 6516-6527, NOV 1 2020.
• Web of Science Citations: 0

Abstract

Melanoma is the most aggressive type of skin cancer and a relevant health problem due to its poor treatment response with high morbidity and mortality rates. This study, aimed to investigate the tissue changes of an improved photodynamic therapy (PDT) response when combined with optical clearing agent (OCA) in the treatment of cutaneous melanoma in mice. Photodithazine (PDZ) was administered intraperitoneally and a solution of OCA was topically applied before PDT irradiation. Due to a resultant refractive index matching, OCA-treated tumors are more optically homogenous, improving the PDT response. Raman analysis revealed, when combined with OCA, the PDT response was more homogenous down to 725 mu m-depth in thickness. (C) 2020 Optical Society of America under the terms of the OSA Open Access Publishing Agreement (AU)

• FAPESP's process: 14/50857-8 - National Institute in Basic Optics and Applied to Life Sciences
• Grantee: Vanderlei Salvador Bagnato
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 13/07276-1 - CEPOF - Optics and Photonic Research Center
• Grantee: Vanderlei Salvador Bagnato
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC