Alternative pressurized liquid extraction using a hard cap espresso machine for determination of polycyclic aromatic hydrocarbons in smoked bacon

Author(s)

Merlo, Thais Cardoso ^[1] ; Molognoni, Luciano ^[2] ; Hoff, Rodrigo Barcellos ^[2] ; Daguer, Heitor ^[2] ; Patinho, Iliani ^[1] ; Contreras-Castillo, Carmen J. ^[1]

Affiliation

^[1] Univ Sao Paulo, Dept Agroind Alimentos & Nutr, Escola Super Agr Luiz de Queiroz, Av Padua Dias 11, Piracicaba, SP - Brazil

^[2] Minist Agr Pecuaria & Abastecimento, Lab Fed Def Agr Secao Lab Avancada Santa Catarina, BR-88102600 Sao Jose, SC - Brazil

• Document type: Journal article
• Source: FOOD CONTROL; v. 120, FEB 2021.
• Web of Science Citations: 0

Abstract

Polycyclic aromatic hydrocarbons (PAHs) are noxious compounds that can be found in some foods, especially those submitted to the traditional smoking process. In this study, a novel pressurized liquid extraction based on hard cap espresso machine followed by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) was developed to determine PAHs in smoked bacon. Method performance was evaluated in accordance to the Commission Decision 2002/657/EC in terms of selectivity, linearity, sensitivity, matrix effect, recovery and detection capability (CCp). Linearity was satisfactory with regression coefficients (R-2) higher than 0.98. Recoveries ranged from 73.9 to 99.9%. CCp values ranged from 0.30 to 0.90 mu g kg(-1), values below the maximum limits suggested by Commission Regulation 835/2011, which propose 2.0 tig kg(-1) for benzo {[}a]pyrene and 12.0 mu g kg(-1) for sum of the four priority PAHs. The method was applied to real samples, with PAHs levels below these limits. The developed method proved to be a fast and cheap approach for the efficient and reliable determination of PAHs in smoked bacon. (AU)

• FAPESP's process: 16/15012-2 - DEVELOPMENT OF CURED AND SMOKED BACONS WITH REFORESTED WOODS: SENSORY CHARACTERIZATION, LIPIDIC CHEMICAL ALTERATIONS AND POLYCYLIC AROMATIC HYDROCARBONS (PAHs) PRODUCTION
• Grantee: Carmen Josefina Contreras Castillo
• Support Opportunities: Regular Research Grants