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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Partial luteolysis during early diestrus in cattle downregulates VEGFA expression and reduces large luteal cell and corpus luteum sizes and plasma progesterone concentration

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Author(s):
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Trevisol, E. [1] ; Mogollon Garcia, H. D. [1] ; Ackermann, C. L. [1] ; Lacerda, W. [2] ; Pires, R. M. L. [2] ; Laufer-Amorin, R. [3] ; Carvalho, R. F. [4] ; Franchi, F. F. [5] ; Castilho, A. C. S. [6] ; Rizzoto, G. [7] ; Kastelic, J. P. [7] ; Ferreira, J. C. P. [1]
Total Authors: 12
Affiliation:
[1] Sao Paulo State Univ UNESP, Sch Vet Med & Anim Sci, Dept Vet Surg & Anim Reprod, BR-18618681 Botucatu, SP - Brazil
[2] APTA SAA, Inst Anim Husb, Nova Odessa, SP - Brazil
[3] Sao Paulo State Univ Unesp, Sch Vet Med & Anim Sci, Dept Vet Clin Sci, BR-18618681 Botucatu, SP - Brazil
[4] Sao Paulo State Univ UNESP, Inst Biosci, Dept Struct & Funct Biol, BR-18618689 Botucatu, SP - Brazil
[5] Sao Paulo State Univ UNESP, Inst Biosci, Dept Pharmacol, BR-18618689 Botucatu, SP - Brazil
[6] Univ Western Sao Paulo Unoeste, BR-9067175 Presidente Prudente, SP - Brazil
[7] Univ Calgary, Fac Vet Med, Dept Prod Anim Hlth, Calgary, AB - Canada
Total Affiliations: 7
Document type: Journal article
Source: Theriogenology; v. 158, p. 188-195, DEC 2020.
Web of Science Citations: 0
Abstract

Our objectives were to investigate potential changes in the size of steroidogenic large luteal cells (LLC) during partial luteolysis induced by a sub-dose of cloprostenol in early diestrus and to determine transcriptional variations in genes involved in corpus luteum (CL) functions. Cows were subjected to an Ovsynch protocol, with the time of the second GnRH treatment defined as Day 0 (D0). On D6, cows were randomly allocated into three treatments: Control (2 mL saline, im; n = 10), 2XPGF (two doses of 500 mu g of cloprostenol, im, 2 h apart; n = 8) or 1/6PGF (single dose of 83.3 mu g of cloprostenol, im; n = 10). Before treatments and every 8 h during the 48-h experimental period, blood samples were collected and CL volumes measured. Furthermore, two CL biopsies were obtained at 24 and 40 h post-treatment. The 1/6PGF treatment caused partial luteolysis, characterized by sudden decreases in plasma progesterone (P4) concentrations, luteal volume and LLC size, followed by increases (to pretreatment values) in P-4 and luteal volume at 24 and 40 h post-treatment, respectively. However, at the end of the study, P4, luteal volume and LLC size were all significantly smaller than in Control cows. Temporally associated with these phenotypes, there was a lower mRNA abundance of VEGFA at 24 and 40 h, and ABCA1 at 24 h (P < 0.05). In conclusion, a sudden reduction in CL size during partial luteolysis induced by a sub-dose of PGF(2 alpha) analog on day 6 of the estrous cycle was attributed to a reduction in LLC size, although these changes did not account for the entire phenomenon. In addition to its involvement in reducing CL size, decreased VEGFA mRNA abundance impaired CL development, resulting in a smaller luteal gland and lower plasma P4 concentrations compared to Control cows. (C) 2020 Elsevier Inc. All rights reserved. (AU)

FAPESP's process: 11/20449-7 - Role of LH during induced luteolysis and characterization of luteal sensitivity to PGF2alpha during estrus cycle and treatment with IFNt
Grantee:Eduardo Trevisol
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 12/18297-7 - Effect of heat stress on follicular development and luteolysis in Honstein cows, role of LH during induced luteolysis and characterization of luteal sensitivity to PGF2± during the estrous cycle
Grantee:João Carlos Pinheiro Ferreira
Support Opportunities: Regular Research Grants